Hammarberg C, Rubio C, Slezak P, Tribukait B, Ohman U
Gut. 1984 Aug;25(8):905-8. doi: 10.1136/gut.25.8.905.
A new approach to the problem of monitoring patients with chronic ulcerative colitis is presented and discussed in connection with a case report. When annual colonoscopies are performed, biopsies are taken for histopathological examination and DNA measurements are made using flow-cytometric techniques (FCM). Using the latter approach, gross chromosomal aberrations indicating malignant transformation in a cell population may be detected. In a 46 year old man with a long history of ulcerative colitis, an area with slight mucosal dysplasia at light microscopy was accompanied by two aneuploid cells lines - that is, colonic mucosa cells with an abnormal amount of DNA in the nuclei. An operation one year later revealed a 5 X 2 mm large adenocarcinoma in the corresponding area of the colon. We suggest that flow-cytometric techniques can be used as a complement to already practised methods for monitoring the colorectal mucosa in colitic patients for the early detection of malignancy.
结合一份病例报告，本文提出并讨论了一种监测慢性溃疡性结肠炎患者的新方法。在进行年度结肠镜检查时，需采集活检组织进行组织病理学检查，并使用流式细胞术（FCM）进行DNA测量。采用后一种方法，可以检测到细胞群体中表明恶性转化的明显染色体畸变。一名患有溃疡性结肠炎病史较长的46岁男性，在光学显微镜下显示轻度黏膜发育异常的区域伴有两个非整倍体细胞系，即细胞核中DNA含量异常的结肠黏膜细胞。一年后进行的手术显示，在结肠相应区域有一个5×2毫米大的腺癌。我们建议，流式细胞术可作为现有监测结肠炎患者结直肠黏膜方法的补充，用于早期发现恶性肿瘤。
