Quirino Lilian Caldas, de Azambuja Carvalho Pedro Henrique, Neto Renato Torres Augusto, Comachio Cássio Amaro, Monteiro Naara Gabriela, Ervolino-Silva Ana Cláudia, Okamoto Roberta, Pereira-Filho Valfrido Antonio
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Rua Humaitá 1680, Araraquara CEP 14801-903, SP, Brazil.
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba CEP 16015-050, SP, Brazil.
Polymers (Basel). 2023 Feb 9;15(4):868. doi: 10.3390/polym15040868.
Guided bone regeneration (GBR) is an approach that induces osteopromotion through the regenerative membranes. These barriers exhibit bioactive behavior and mechanical function. Polydioxanone is a synthetic option, already used in medicine and dentistry, with good results in bone regeneration. This study aimed to evaluate bone repair in critical defects in rat calvaria using a polydioxanone membrane (Plenum Guide) compared with a commercially available collagen-based membrane (Bio-Gide). The bone defects were filled with Plenum Oss, a synthetic bone graft, hydroxyapatite:β-tricalcium phosphate, 70:30%, Group PG (Plenum Guide + Plenum Oss), and Group BG (Geistlich Bio-Gide + Plenum Oss). The specimens were submitted to immunohistochemical (RUNX2 and OPN), gene expression (RUNX2, IBSP, and VEGF), histometric, and microtomography analyses after 07, 15, 30, and 60 days postoperative. PG group showed greater immunolabeling area for RUNX2 and OPN, higher gene expression of VEGF (3.15 ± 0.85), and IBSP (24.9 ± 0.59). However, there was no statistical difference between groups in the histometric analysis regarding the percentage of connective tissue PG (0.83 ± 0.45), BG (0.70 ± 0.34), neoformed bone PG (0.60 ± 0.4), BG (0.65 ± 0.51), and remaining biomaterial PG (0.84 ± 0.31), BG (0.91 ± 0.33). In addition, there was no statistical difference between groups by micro-CT analysis. The absorbable-synthetic membrane, Plenum Guide, is an effective membrane for guided bone regeneration.
引导骨再生（GBR）是一种通过再生膜诱导骨促进的方法。这些屏障具有生物活性行为和机械功能。聚二氧六环酮是一种已用于医学和牙科的合成材料，在骨再生方面取得了良好效果。本研究旨在评估使用聚二氧六环酮膜（Plenum Guide）与市售胶原基膜（Bio-Gide）相比，对大鼠颅骨临界缺损的骨修复情况。骨缺损用合成骨移植材料Plenum Oss（羟基磷灰石：β-磷酸三钙，70:30%）填充，分为PG组（Plenum Guide + Plenum Oss）和BG组（Geistlich Bio-Gide + Plenum Oss）。术后07、15、30和60天，对标本进行免疫组织化学（RUNX2和OPN）、基因表达（RUNX2、IBSP和VEGF）、组织计量学和显微断层扫描分析。PG组RUNX2和OPN的免疫标记面积更大，VEGF（3.15±0.85）和IBSP（24.9±0.59）的基因表达更高。然而，在组织计量学分析中，PG组（0.83±0.45）和BG组（0.70±0.34）的结缔组织百分比、PG组（0.60±0.4）和BG组（0.65±0.51）的新形成骨百分比以及PG组（0.84±0.31）和BG组（0.91±0.33）的剩余生物材料百分比在两组之间没有统计学差异。此外，通过显微CT分析，两组之间也没有统计学差异。可吸收合成膜Plenum Guide是引导骨再生的有效膜。
