School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730030, China.
School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
Genes (Basel). 2022 Dec 25;14(1):64. doi: 10.3390/genes14010064.
Turcz, a perennial herb of the Asteraceae family, is one of the horticultural and medicinal plants used for curing various diseases and is widely distributed in China and other Asian countries. It possesses antibacterial, antimetastatic, antiangiogenic, and antioxidant properties along with anticancer potential. However, the intrageneric classification and phylogenetic relationships within have long been controversial due to the lack of high-resolution molecular markers, and the complete chloroplast (cp) genome sequencing has not been reported with new evolutionary insights. In the present study, was used as an experimental material, and its genome was sequenced using high-throughput sequencing technology. We assembled the complete cp genome, and a systematic analysis was conducted for , acquiring the correspondence of its NCBI accession number (OK545755). The results showed that the cp genome of is a typical tetrad structure with a total length of 152,401 bp, and the genome encodes 133 genes. Analysis of the complete cp genomes of 20 Eupatorieae shows that the number of simple sequence repeats (SSRs) ranged from 19 to 36 while the number of long sequence repeats was 50 in all cases. Eleven highly divergent regions were identified and are potentially useful for the DNA barcoding of Eupatorieae. Phylogenetic analysis among 22 species based on protein-coding genes strongly supported that is more closely related to and belongs to the same branch. The genome assembly and analysis of the cp genome of will facilitate the identification, taxonomy, and utilization of as well as provide more accurate evidence for the taxonomic identification and localization of Asteraceae plants.
Turcz 是菊科的一种多年生草本植物，是用于治疗各种疾病的园艺和药用植物之一，广泛分布于中国和其他亚洲国家。它具有抗菌、抗转移、抗血管生成和抗氧化特性，同时具有抗癌潜力。然而，由于缺乏高分辨率的分子标记，长期以来 属内的分类和系统发育关系一直存在争议，并且尚未报道其完整的叶绿体（cp）基因组测序以提供新的进化见解。在本研究中，以 为实验材料，使用高通量测序技术对其基因组进行了测序。我们组装了完整的 cp 基因组，并对其进行了系统分析，获得了其在 NCBI 中的登录号（OK545755）的对应关系。结果表明， 的 cp 基因组为典型的四联体结构，全长 152401bp，基因组共编码 133 个基因。对 20 种泽兰属 cp 基因组的全面分析表明，简单序列重复（SSR）的数量范围为 19 到 36，而长序列重复的数量在所有情况下均为 50。鉴定出 11 个高度分化区域，这些区域可能对泽兰属的 DNA 条形码有用。基于蛋白质编码基因的 22 种物种的系统发育分析强烈支持 与 和 关系更密切，属于同一分支。 cp 基因组的组装和分析将有助于 的确切鉴定、分类和利用，并为菊科植物的分类鉴定和定位提供更准确的证据。
