Trakarnsanga Kongtana, Vorasan Nutchavadee, Tantarungsee Nutnicha, Wattanapanitch Methichit, Vatanashevanopakorn Chinnavuth, Suktitipat Bhoom, Metheetrairut Chanatip
Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Genetic Epidemiology Research Group, Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
BMC Res Notes. 2025 Aug 17;18(1):356. doi: 10.1186/s13104-025-07437-3.
Induced pluripotent stem cells (iPSCs) are generated from a vast number of adult cell types. While they all acquired embryonic stem cell (ESC)-like properties during reprogramming, differences in certain characteristics, including differentiation potential, remained. These differences are hypothesized to be due to epigenetic memory or individual genetic background.
This study compared microRNA (miRNA) profiles, which is one aspect of epigenetic regulation, of four iPSC lines derived from dermal fibroblasts, hair follicle keratinocytes, peripheral blood T cells, and exfoliated renal epithelial cells by nCounter miRNA expression panels. 110 miRNAs were expressed in all iPSC lines, which accounted for 67.5-75.9% of expressed miRNAs in each line, while there were only 3, 4, 9, and 28 uniquely expressed miRNAs in each line. MiRNA profiles of the four iPSC lines clustered together, but away from those of mature cells of origin or ESCs retrieved from the Gene Expression Omnibus database as analyzed by principal component analysis. These results suggested that the miRNA profiles of the four iPSC lines are mostly similar to each other, though with some specific unique gene expression, and do not appear to indicate any obvious differences between the iPSC lines derived from different adult cell types in terms of miRNA expression.
诱导多能干细胞（iPSC）可从大量成年细胞类型中产生。虽然它们在重编程过程中都获得了类似胚胎干细胞（ESC）的特性，但在某些特征方面仍存在差异，包括分化潜能。这些差异被认为是由于表观遗传记忆或个体遗传背景所致。
本研究通过nCounter miRNA表达分析板比较了源自真皮成纤维细胞、毛囊角质形成细胞、外周血T细胞和脱落肾上皮细胞的4种iPSC系的miRNA谱，这是表观遗传调控的一个方面。所有iPSC系中均表达了110种miRNA，占各系中表达miRNA的67.5 - 75.9%，而各系中分别仅有3、4、9和28种独特表达的miRNA。通过主成分分析，这4种iPSC系的miRNA谱聚集在一起，但与从基因表达综合数据库中检索到的起源成熟细胞或ESC的miRNA谱不同。这些结果表明，这4种iPSC系的miRNA谱大多彼此相似，尽管存在一些特定的独特基因表达，并且在miRNA表达方面，似乎并未表明源自不同成年细胞类型的iPSC系之间存在任何明显差异。
