Wang Pengbo, Wan Dongman, Peng Ting, Yang Yujing, Wen Xuefang, Yan Xianghui, Xia Jiaheng, Zhu Qingwen, Yu Ping, Gong Deming, Zeng Zheling
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Jiangxi Province Key Laboratory of Edible and Medicinal Resources Exploitation, Nanchang University, Nanchang 330031, China.
Foods. 2023 Jan 8;12(2):293. doi: 10.3390/foods12020293.
seed kernel oil (CCSKO) is one of the important natural medium chain triglycerides (MCT) resources, with more than 95.00% of medium chain fatty acids found in the world, and has various physiological effects. However, CCSKO has not been generally recognized as a safe oil or new food resource yet. The acute oral toxicity test and a standard battery of genotoxicity tests (mammalian erythrocyte micronucleus test, Ames test, and in vitro mammalian cell TK gene mutation test) of CCSKO as a new edible plant oil were used in the study. The results of the acute oral toxicity test showed that CCSKO was preliminary non-toxic, with an LD value higher than 21.5 g/kg body weight. In the mammalian erythrocyte micronucleus test, there was no concentration-response relationship between the dose of CCSKO and micronucleus value in polychromatic erythrocytes compared to the negative control group. No genotoxicity was observed in the Ames test in the presence or absence of S9 at 5000 μg/mL. In vitro mammalian cell TK gene mutation test showed that CCSKO did not induce in vitro mammalian cell TK gene mutation in the presence or absence of S9 at 5000 μg/mL. These results indicated that CCSKO is a non-toxic natural medium-chain oil.
籽仁油（CCSKO）是重要的天然中链甘油三酯（MCT）资源之一，其含有的中链脂肪酸在世界范围内超过95.00%，并且具有多种生理作用。然而，CCSKO尚未被普遍认定为安全油脂或新型食品资源。本研究采用CCSKO作为新型食用植物油进行急性经口毒性试验以及一系列标准的遗传毒性试验（哺乳动物红细胞微核试验、Ames试验和体外哺乳动物细胞TK基因突变试验）。急性经口毒性试验结果表明，CCSKO初步判定为无毒，其LD值高于21.5 g/kg体重。在哺乳动物红细胞微核试验中，与阴性对照组相比，CCSKO剂量与多染红细胞微核值之间无浓度-反应关系。在5000 μg/mL时，无论有无S9，Ames试验均未观察到遗传毒性。体外哺乳动物细胞TK基因突变试验表明，在5000 μg/mL时，无论有无S9，CCSKO均未诱导体外哺乳动物细胞TK基因突变。这些结果表明，CCSKO是一种无毒的天然中链油脂。
