Loyola Khanty, Karsulovic Claudio, Cabrera Raúl, Perez Claudio, Hojman Lía
Dermatology Section, Surgery Department, Facultad de Medicina Clínica Alemana de Santiago, Universidad del Desarrollo, Santiago P.O. Box 7630000, Chile.
Rheumatology Section, Internal Medicine Department, Facultad de Medicina Clínica Alemana de Santiago, Universidad del Desarrollo, Santiago P.O. Box 7630000, Chile.
Metabolites. 2023 Jan 11;13(1):116. doi: 10.3390/metabo13010116.
Psoriasis is a skin disease with occasional involvement of non-cutaneous territories. Beyond the usual, cardiovascular events are more frequent in these patients and correlate only partially with disease activity, suggesting the presence of other unknown factors. We selected ten psoriatic patients without treatment in the last year and matched them for age and gender with eleven healthy subjects. Ficoll-extracted mononuclear cells were analyzed with flow cytometry for monocyte surface phenotype markers, intracellular NFκB/inflammasome-dependent interleukins, and chemotaxis receptor CXCR3. Using ELISA, patient serum was evaluated for ADAMTS7 and CXCL10. Inflammatory M1 monocytes showed higher levels of IL-1β and IL-6 in psoriatic patients. M2 monocytes also showed higher levels of intracellular inflammatory cytokines. Nevertheless, IL-6 values were higher compared to other monocytes and IL-1β. The mTORC activation markers ADAMTS7 and S6Rp were higher in psoriatic patients than in healthy controls. In psoriatic patients, serum levels of ADAMTS7 were elevated, and M2 monocytes showed a distinct inflammatory response with higher relative levels of NFκB-dependent IL-6 and less activity of the CXCR3-CXCL10 chemotactic pathway. These data suggest pathways with potential markers for prediction and early detection of cardiovascular risk in psoriatic patients.
银屑病是一种偶尔累及非皮肤区域的皮肤病。除了常见情况外，这些患者心血管事件更为频繁，且仅部分与疾病活动相关，提示存在其他未知因素。我们选择了去年未接受治疗的10例银屑病患者，并根据年龄和性别将他们与11名健康受试者进行匹配。采用流式细胞术分析Ficoll分离的单核细胞的单核细胞表面表型标志物、细胞内NFκB/炎性小体依赖性白细胞介素和趋化因子受体CXCR3。使用酶联免疫吸附测定法（ELISA）评估患者血清中的ADAMTS7和CXCL10水平。炎症性M1单核细胞在银屑病患者中显示出较高水平的IL-1β和IL-6。M2单核细胞也显示出较高水平的细胞内炎性细胞因子。然而，与其他单核细胞相比，IL-6值更高，且IL-1β也是如此。银屑病患者中mTORC激活标志物ADAMTS7和S6Rp高于健康对照。在银屑病患者中，ADAMTS7血清水平升高，M2单核细胞表现出独特的炎症反应，NFκB依赖性IL-6相对水平较高，而CXCR3-CXCL10趋化途径活性较低。这些数据提示了银屑病患者心血管风险预测和早期检测的潜在标志物途径。
