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药用植物的细菌群落:结构特征与根际效应

Bacterial Communities of L. Medicinal Plants: Structural Features and Rhizosphere Effect.

作者信息

Zharkova Ekaterina K, Vankova Anna A, Selitskaya Olga V, Malankina Elena L, Drenova Natalya V, Zhelezova Alena D, Khlyustov Vitaliy K, Belopukhov Sergey L, Zhevnerov Aleksey V, Sviridova Ludmila A, Fomina Tatiana N, Kozlov Andrey V

机构信息

Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow 127434, Russia.

All-Russian Plant Quarantine Center, Pogranichnaya St. 32, Bykovo, Ramensky District, Moscow 140150, Russia.

出版信息

Microorganisms. 2023 Jan 12;11(1):197. doi: 10.3390/microorganisms11010197.

Abstract

Bacterial communities associated with medicinal plants are an essential part of ecosystems. The rhizosphere effect is rather important in the cultivation process. The purpose of the study was to analyze the rhizosphere effect of oregano ( L.), peppermint ( L.), thyme ( L.), creeping thyme ( L.) and sage ( L.). To estimate the quantity of 16S bacteria ribosomal genes, qPCR assays were used. To compare bacterial communities' structure of medicinal plants rhizosphere with bulk soil high-throughput sequencing of the 16S rRNA targeting variable regions V3-V4 of bacteria was carried out. The highest bacterial abundance was associated with L., L. and L., and the lowest was associated with the L. rhizosphere. Phylum was predominant in all rhizosphere samples. The maximum bacterial α-diversity was found in L. rhizosphere. According to bacterial β-diversity calculated by the Bray-Curtis metric, L. root zone significantly differed from bulk soil. The rhizosphere effect was positive to the , , , and .

摘要

与药用植物相关的细菌群落是生态系统的重要组成部分。根际效应在栽培过程中相当重要。本研究的目的是分析牛至(唇形科)、薄荷(唇形科)、百里香(唇形科)、匐枝百里香(唇形科)和鼠尾草(唇形科)的根际效应。为了估计16S细菌核糖体基因的数量,使用了定量聚合酶链反应(qPCR)分析。为了比较药用植物根际细菌群落结构与原状土,对细菌16S rRNA靶向可变区V3 - V4进行了高通量测序。最高的细菌丰度与牛至、薄荷和百里香相关,最低的与匐枝百里香根际相关。变形菌门在所有根际样品中占主导地位。在薄荷根际发现了最大的细菌α多样性。根据通过Bray - Curtis度量计算的细菌β多样性,薄荷根区与原状土有显著差异。根际效应对于牛至、薄荷、百里香、匐枝百里香和鼠尾草是积极的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5dd/9865931/470d7fb0ef42/microorganisms-11-00197-g001.jpg

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