Cui Zhijia, Li Ran, Li Fan, Jin Ling, Wu Haixu, Cheng Chunya, Ma Yi, Wang Zhenheng, Wang Yuanyuan
College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, Gansu, China.
Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-Constructed by Gansu Province & MOE of PRC, Lanzhou, Gansu, China.
Front Microbiol. 2023 Feb 17;14:1070815. doi: 10.3389/fmicb.2023.1070815. eCollection 2023.
Maxim. is a Chinese endemic species with high medicinal value distributed in the northeastern part of the Tibetan Plateau. root-associated rhizosphere bacterial communities shaped by soil properties may maintain the stability of soil structure and regulate growth, but the rhizosphere bacterial community structure of wild from natural populations is not clear.
In the current study, soil samples from 12 sites within the natural range of wild were collected to investigate the compositions of bacterial communities high-throughput sequencing of 16S rRNA genes and multivariate statistical analysis combined with soil properties and plant phenotypic characteristics.
Bacterial communities varied between rhizosphere and bulk soil, and also between sites. Co-occurrence networks were more complex in rhizosphere soil (1,169 edges) than in bulk soil (676 edges). There were differences in bacterial communities between regions, including diversity and composition. Proteobacteria (26.47-37.61%), Bacteroidetes (10.53-25.22%), and Acidobacteria (10.45-23.54%) were the dominant bacteria, and all are associated with nutrient cycling. In multivariate statistical analysis, both soil properties and plant phenotypic characteristics were significantly associated with the bacterial community ( < 0.05). Soil physicochemical properties accounted for most community differences, and pH was a key factor ( < 0.01). Interestingly, when the rhizosphere soil environment remained alkaline, the C and N contents were lowest, as was the biomass of the medicinal part bulb. This might relate to the specific distribution of genera, such as , , (relative abundance >0.01), which all have significantly correlated with the biomass of ( < 0.05).
is evidently averse to alkaline soil with high potassium contents, but this requires future verification. The results of the present study may provide theoretical guidance and new insights for the cultivation and domestication of .
羌活是中国特有的物种，具有很高的药用价值，分布于青藏高原东北部。受土壤性质影响的根际细菌群落可能维持土壤结构的稳定性并调节其生长，但来自自然种群的野生羌活的根际细菌群落结构尚不清楚。
在本研究中，采集了野生羌活自然分布范围内12个地点的土壤样本，通过16S rRNA基因高通量测序和多变量统计分析，并结合土壤性质和植物表型特征，研究细菌群落的组成。
根际土壤和非根际土壤之间以及不同地点之间的细菌群落存在差异。根际土壤中的共现网络（1169条边）比非根际土壤（676条边）更复杂。不同区域的细菌群落在多样性和组成上存在差异。变形菌门（26.47%-37.61%）、拟杆菌门（10.53%-25.22%）和酸杆菌门（10.45%-23.54%）是优势细菌，且均与养分循环相关。在多变量统计分析中，土壤性质和植物表型特征均与细菌群落显著相关（P<0.05）。土壤理化性质解释了大部分群落差异，pH是关键因素（P<0.01）。有趣的是，当根际土壤环境保持碱性时，碳和氮含量最低，药用部位鳞茎的生物量也最低。这可能与某些属的特定分布有关，如羌活属、独活属、囊瓣芹属（相对丰度>0.01），它们均与羌活的生物量显著相关（P<0.05）。
羌活显然不适应高钾碱性土壤，但这需要未来进一步验证。本研究结果可为羌活的栽培和驯化提供理论指导和新的见解。
