Moussa Zeiad, Alanazi Yasmene F, Khateb Aiah Mustafa, Eldadamony Noha M, Ismail Marwa M, Saber WesamEldin I A, Darwish Doaa Bahaa Eldin
Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt.
Department of Biochemistry, Faculty of Science, University of Tabuk, Tabuk 71421, Saudi Arabia.
Microorganisms. 2023 Jan 12;11(1):198. doi: 10.3390/microorganisms11010198.
The beneficial microorganisms represent a new and hopeful solution for a sustainable environment and development. In this investigation, ZNW, isolated from seeds, was domiciliated within the pea plant for improving growth, disease management, and enhancement of productivity. NZW was isolated from deformed pea seeds, representing the first record of the pathogen caused by pea damping-off. Both fungi were molecularly identified. ZNW produced several lytic enzymes and bioactive metabolites as detected by GC-MC. The SEM illustrated the mycoparasitic behavior of ZNW on NZW mycelia. In the pot experiment, domiciliated the root and grew as an endophytic fungus, leading to root vessel lignification. Under soil infection, reduced damping-off, by enhancing peroxidase, polyphenol, total phenols, and photosynthetic pigments content. The vegetative growth, yield, and soil dehydrogenase activity were improved, with an enhancement in the numerical diversity of the microbial rhizosphere. This work may enable more understanding of the plant-fungal interaction, yet, working on domiciliation is recommended as a new approach to plant protection and growth promotion under various ecological setups.
有益微生物为可持续环境与发展提供了一种全新且充满希望的解决方案。在本研究中，从种子中分离出的ZNW在豌豆植株内定殖，以促进生长、防治病害并提高产量。NZW是从畸形豌豆种子中分离得到的，它是导致豌豆猝倒病的病原体的首次记录。对这两种真菌都进行了分子鉴定。通过气相色谱 - 质谱联用（GC - MC）检测发现，ZNW能产生多种裂解酶和生物活性代谢产物。扫描电子显微镜（SEM）显示了ZNW对NZW菌丝体的重寄生行为。在盆栽试验中，ZNW定殖于根部并作为内生真菌生长，导致根导管木质化。在土壤感染情况下，通过提高过氧化物酶、多酚、总酚和光合色素含量，减少了猝倒病的发生。改善了营养生长、产量和土壤脱氢酶活性，同时提高了根际微生物的数值多样性。这项工作可能有助于更深入理解植物 - 真菌之间的相互作用，然而，建议将定殖研究作为在各种生态环境下植物保护和促进生长的一种新方法。
