Đorđevski Nikoleta, Uba Abdullahi Ibrahim, Zengin Gokhan, Božunović Jelena, Gašić Uroš, Ristanović Elizabeta, Ćirić Ana, Nikolić Biljana, Stojković Dejan
Laboratory of Immunology, Institute of Microbiology, Medical Military Academy, Crnotravska 17, 11000 Belgrade, Serbia.
Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Kadir Has University, 34083 Istanbul, Turkey.
Pharmaceuticals (Basel). 2022 Dec 23;16(1):21. doi: 10.3390/ph16010021.
This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3--glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of with contemporary in vitro scientific approaches.
本研究旨在调查不同提取溶剂系统对巴尔干地区中世纪传统药用植物石蒜（石蒜科）的化学成分和生物活性的影响。通过超高效液相色谱（-）高分辨电喷雾电离串联四极杆飞行时间质谱（UHPLC(-)HESI-QqQ-MS/MS）对九种不同提取物进行了靶向化学分析。对从感染的人类皮肤和角膜组织中分离出的16株临床细菌、酵母和皮肤真菌分离株研究了提取物的抗菌和抗生物膜活性。在人永生化角质形成细胞（HaCaT细胞系）上测试了细胞毒性和伤口愈合特性。通过六种不同的测定方法评估了抗氧化活性，同时在选定的酶抑制测定结合分子建模中确定了对某些神经退行性疾病和2型糖尿病的有益潜力。结果表明，所获得的提取物富含酚类化合物，尤其是黄酮苷，如芦丁和山奈酚3-O-葡萄糖苷。所有提取物均表现出抗菌、伤口愈合、抗氧化和抗酶特性。本研究首次将野生花卉的中世纪药用与当代体外科学方法联系起来。
