Petrović Jovana D, Carević Milićević Tamara A, Glamočlija Jasmina M, Kulaš Jelena B, Mirkov Ivana I
Institute for Biological Research "Siniša Stanković", National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia.
Pharmaceuticals (Basel). 2025 Mar 15;18(3):416. doi: 10.3390/ph18030416.
This study explores wound healing and the antimicrobial potential of a natural formulation containing a polysaccharide extract from , hyaluronic acid, and dexpanthenol. Wound healing effects were assessed using HaCaT keratinocytes, while antimicrobial activity was evaluated against human skin pathogens using a microdilution assay. In vitro cytotoxicity tests ensured formulation safety, whereas in vivo wound healing was further investigated using an animal model. Gene expression analysis was performed to assess the molecular mechanisms involved. The unique glucan composition of (15.38% α-glucans and 7.91% β-glucans) deviated from typical mushroom polysaccharide profiles, warranting further exploration of its bioactivity. In vitro mushroom polysaccharides promoted 25.35% wound closure after 24 hours, while the three-component formulation achieved 35.81% closure. Antibacterial activity showed a minimum inhibitory concentration (MIC) of 0.44-1.75 mg/mL and minimum bactericidal concentration (MBCs) of 0.88-3.50 mg/mL, while antifungal activity ranged from 0.22 to 0.44 mg/mL (MICs) and 0.44 to 0.88 mg/mL (minimum fungicidal concentration-MFC). In vivo data showed that 60% of treated wounds fully closed by day 11, despite no statistically significant difference from the control. However, gene expression analysis highlighted VEGF and collagen upregulation, indicating an enhancement of wound healing on a molecular level. The novel three-component formulation demonstrated consistent wound healing and antimicrobial properties, supporting its potential as a safe and effective treatment for chronic and acute wounds.
本研究探讨了一种含有[具体来源]多糖提取物、透明质酸和泛醇的天然制剂的伤口愈合作用及抗菌潜力。使用HaCaT角质形成细胞评估伤口愈合效果，同时采用微量稀释法评估其对人类皮肤病原体的抗菌活性。体外细胞毒性试验确保了制剂的安全性，而使用动物模型进一步研究了体内伤口愈合情况。进行基因表达分析以评估其中涉及的分子机制。[具体来源]独特的葡聚糖组成（15.38%的α-葡聚糖和7.91%的β-葡聚糖）与典型的蘑菇多糖谱不同，这值得进一步探索其生物活性。体外试验中，蘑菇多糖在24小时后促进了25.35%的伤口闭合，而三元制剂实现了35.81%的闭合。抗菌活性显示最低抑菌浓度（MIC）为0.44 - 1.75 mg/mL，最低杀菌浓度（MBC）为0.88 - 3.50 mg/mL，而抗真菌活性范围为0.22至0.44 mg/mL（MIC）和0.44至0.88 mg/mL（最低杀真菌浓度 - MFC）。体内数据显示，尽管与对照组无统计学显著差异，但60%的治疗伤口在第11天完全闭合。然而，基因表达分析突出了血管内皮生长因子（VEGF）和胶原蛋白的上调，表明在分子水平上伤口愈合得到增强。这种新型三元制剂表现出一致的伤口愈合和抗菌特性，支持其作为慢性和急性伤口安全有效治疗方法的潜力。
