Department of Botany, Faculty of Science, Sudan University of Science and Technology (SUST), Khartoum P.O. Box 407, Sudan.
Department of Botany, Faculty of Science, University of Khartoum, Khartoum P.O. Box 321, Sudan.
Molecules. 2022 Aug 30;27(17):5590. doi: 10.3390/molecules27175590.
Several species within the genera or have a treasure of traditional medicines worldwide and can be a promising source of bioactive molecules. The objective of the present study was to evaluate the phenolic content and antioxidant and enzyme inhibition activities of leaf methanolic extracts of L., L., Mill., and Mill. The two spp. contained higher total polyphenolic content (42.23-49.75 mg GAE/g) than the two spp., and had significantly ( ˂ 0.05) the highest concentration. On the other hand, the spp. showed higher total flavonoid content (41.47-59.24 mg rutin equivalent per g of extract) than that found in the two spp., and significantly ( ˂ 0.05) accumulated the highest amount. HPLC-MS/MS analysis of 38 selected bioactive compounds showed that the majority of compounds were identified in the four species, but with sharp variations in their concentrations. was dominated by epicatechin (8928.75 µg/g), by kaempferol-3-glucoside (47,360.04 µg/g), while rutin was the major compound in (17,285.02 µg/g) and (6381.85). The methanolic extracts of the two species exerted significantly ( ˂ 0.05) higher antiradical activity, metal reducing capacity, and total antioxidant activity than that recorded from the two species' methanolic extracts, and displayed significantly ( ˂ 0.05) the highest values. significantly ( ˂ 0.05) exhibited the highest metal chelating power. The results of the enzyme inhibition activity showed that the four species possessed anti-AChE activity, and the highest value, but not significantly ( ≥ 0.05) different from those obtained by the two spp., was exerted by . . The spp. exhibited significantly ( ˂ 0.05) higher anti-BChE and anti-Tyr properties than the spp., and revealed significantly ( ˂ 0.05) the highest values. revealed significantly ( ˂ 0.05) the highest α- amylase inhibition, while the four species had more or less the same effect against the α-glucosidase enzyme. Multivariate analysis and in silico studies showed that many of the identified phenols may play key roles as antioxidant and enzyme inhibitory properties. Thus, these and species could be a promising source of natural bioactive agents with beneficial effects for human health.
几种 属和 属植物的叶子甲醇提取物具有丰富的传统药物，可能是生物活性分子的有前途的来源。本研究的目的是评估 L.、 L.、 Mill. 和 Mill. 的叶甲醇提取物的总酚含量、抗氧化和酶抑制活性。两种 种植物的总多酚含量（42.23-49.75mg GAE/g）均高于两种 种植物， 种植物的含量显著（ ˂ 0.05）最高。另一方面，两种 种植物的总类黄酮含量（41.47-59.24mg 芦丁当量/g 提取物）高于两种 种植物， 种植物的含量显著（ ˂ 0.05）最高。对 38 种选定生物活性化合物的 HPLC-MS/MS 分析表明，大多数化合物在四种物种中均被鉴定，但浓度差异很大。 以表儿茶素（8928.75µg/g）为主， 以山奈酚-3-葡萄糖苷（47,360.04µg/g）为主，而芦丁是 （17,285.02µg/g）和 （6381.85）的主要化合物。两种 种植物的甲醇提取物表现出显著（ ˂ 0.05）更高的自由基清除活性、金属还原能力和总抗氧化活性，而两种 种植物的甲醇提取物则表现出显著（ ˂ 0.05）更高的活性， 表现出显著（ ˂ 0.05）更高的金属螯合能力。酶抑制活性结果表明，四种植物均具有抗 AChE 活性，最高值与两种 种植物的活性值无显著差异（≥0.05），但 。两种 种植物表现出显著（ ˂ 0.05）更高的抗 BChE 和抗 Tyr 活性， 种植物的活性值显著（ ˂ 0.05）最高。 表现出显著（ ˂ 0.05）更高的α-淀粉酶抑制作用，而四种植物对α-葡萄糖苷酶的抑制作用大致相同。多元分析和计算机模拟研究表明，许多鉴定出的酚类物质可能在抗氧化和酶抑制特性方面发挥关键作用。因此，这些 和 种植物可能是具有有益人类健康的天然生物活性物质的有前途的来源。
