Wu Yun-Zhen, Tsai Yen-Yu, Chang Long-Sen, Chen Ying-Jung
Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
Pharmaceuticals (Basel). 2021 Oct 22;14(11):1071. doi: 10.3390/ph14111071.
Hyperglycemic environment-induced oxidative stress-mediated matrix metalloproteinase-1 (MMP-1) plays a crucial role in the degradation of the extracellular matrix (ECM), which might contribute to premature skin aging. Synthesized, environmentally friendly gallic acid-coated gold nanoparticles (GA-AuNPs) have been evaluated as an anti-aging antioxidant. Their microstructure was characterized by transmission electron microscopy (TEM), which showed that GA-AuNPs are spherical when prepared at pH 11. Dynamic light scattering (DLS) analysis revealed that the average hydrodynamic diameter of a GA-AuNP is approximately 40 nm and with a zeta potential of -49.63 ± 2.11 mV. Additionally, the present data showed that GA-AuNPs have a superior ability to inhibit high glucose-mediated MMP-1-elicited type I collagen degradation in dermal fibroblast cells. Collectively, our data indicated that high-glucose-mediated ROS production was reduced upon cell treatment with GA-AuNPs, which blocked p38 MAPK/ERK-mediated c-Jun, c-Fos, ATF-2 phosphorylation, and the phosphorylation of NFκB, leading to the down-regulation of MMP-1 mRNA and protein expression in high glucose-treated cells. Our findings suggest that GA-AuNPs have a superior ability to inhibit high-glucose-mediated MMP-1-elicited ECM degradation, which highlights its potential as an anti-aging ingredient.
高血糖环境诱导的氧化应激介导的基质金属蛋白酶-1（MMP-1）在细胞外基质（ECM）降解中起关键作用，这可能导致皮肤过早老化。已评估合成的、环境友好的没食子酸包被的金纳米颗粒（GA-AuNPs）作为一种抗衰老抗氧化剂。通过透射电子显微镜（TEM）对其微观结构进行了表征，结果表明在pH 11条件下制备的GA-AuNPs呈球形。动态光散射（DLS）分析显示，GA-AuNP的平均流体动力学直径约为40 nm，zeta电位为-49.63±2.11 mV。此外，目前的数据表明，GA-AuNPs具有卓越的能力，可抑制高糖介导的MMP-1诱导的真皮成纤维细胞中I型胶原蛋白的降解。总体而言，我们的数据表明，用GA-AuNPs处理细胞后，高糖介导的活性氧生成减少，这阻断了p38 MAPK/ERK介导的c-Jun、c-Fos、ATF-2磷酸化以及NFκB的磷酸化，导致高糖处理细胞中MMP-1 mRNA和蛋白表达下调。我们的研究结果表明，GA-AuNPs具有卓越的能力，可抑制高糖介导的MMP-1诱导的ECM降解，这突出了其作为抗衰老成分的潜力。
