Ivesic Caroline, Krammer Stefanie, Koller-Peroutka Marianne, Laarouchi Aicha, Gruber Daniela, Lang Ingeborg, Lichtscheidl Irene K, Adlassnig Wolfram
Functional and Evolutionary Ecology, Faculty of Life Sciences, University of Vienna, Djerassiplatz 1, 1030 Vienna, Austria.
Core Facility Cell Imaging and Ultrastructure Research, Faculty of Life Sciences, University of Vienna, Djerassiplatz 1, 1030 Vienna, Austria.
Plants (Basel). 2023 Jan 11;12(2):341. doi: 10.3390/plants12020341.
Carnivorous plants adsorb prey-derived nutrients partly by endocytosis. This study quantifies endocytosis in Drosophyllum lusitanicum, Drosera capensis, Drosera roseana, Dionaea muscipula and Nepenthes × ventrata. Traps were exposed to 1% fluorescent-labeled albumin (FITC-BSA), and uptake was quantified repeatedly for 64 h. Formation of vesicles started after ≤1 h in adhesive traps, but only after 16 h in species with temporary stomach (D. muscipula and N. × ventrata). In general, there are similarities in the observed species, especially in the beginning stages of endocytosis. Nonetheless, further intracellular processing of endocytotic vesicles seems to be widely different between species. Endocytotic vesicle size increased significantly over time in all species except in D. capensis. Fluorescence intensity of the endocytotic vesicles increased in all species except D. muscipula. After 64 h, estimates for FITC-BSA absorption per gland ranged from 5.9 ± 6.3 ng in D. roseana to 47.8 ± 44.3 ng in N. × ventrata, demonstrating that endocytosis substantially contributes to the adsorption of prey-derived nutrients.
食肉植物部分通过胞吞作用吸收猎物来源的养分。本研究对葡萄牙茅膏菜、海角茅膏菜、玫瑰茅膏菜、捕蝇草和红瓶猪笼草中的胞吞作用进行了量化。将捕虫器暴露于1%的荧光标记白蛋白（异硫氰酸荧光素-牛血清白蛋白）中，并在64小时内反复对摄取量进行量化。在粘性捕虫器中，囊泡在≤1小时后开始形成，但在具有临时消化腔的物种（捕蝇草和红瓶猪笼草）中，囊泡在16小时后才开始形成。一般来说，所观察的物种之间存在相似性，尤其是在胞吞作用的初始阶段。尽管如此，胞吞囊泡的进一步细胞内处理在不同物种之间似乎有很大差异。除了海角茅膏菜外，所有物种的胞吞囊泡大小均随时间显著增加。除捕蝇草外，所有物种的胞吞囊泡荧光强度均增加。64小时后，每个腺体对异硫氰酸荧光素-牛血清白蛋白的吸收量估计值在玫瑰茅膏菜中为5.9±6.3纳克，在红瓶猪笼草中为47.8±44.3纳克，这表明胞吞作用对猎物来源养分的吸收有很大贡献。
