Janik A, Juni E, Heym G A
J Clin Microbiol. 1976 Jul;4(1):71-81. doi: 10.1128/jcm.4.1.71-81.1976.
A rapid method for the detection of Neisseria gonorrhoeae, making use of the ability of deoxyribonucleic acid samples from clinically isolated strains of this organism to transform nutritional mutants of a particular strain of N. gonorrhoeae, has been described. In addition to using isolated cultures, transforming deoxyribonucleic acid can be obtained directly from the material that adheres to swabs of the cervix or the urethra. The time interval for transfer of swabs to the diagnostic laboratory is not a significant factor. It is not necessary to use pure cultures on primary isolation plates to obtain definitive results. Nongonorrhoeae neisserias, as well as a large variety of commonly encountered unrelated bacteria, do not react or interfere in the transformation assay when using one of the mutant strains under a standardized set of conditions. The entire assay can be completed in less than 24 h. It has also been shown that type T4 cells of the strain of N. gonorrhoeae employed in the present study are competent for genetic transformation, although type T4 cells are transformed at a significantly lower frequency than are type T2 cells of the same strain.
一种利用临床分离的淋病奈瑟菌菌株的脱氧核糖核酸样本转化特定淋病奈瑟菌菌株营养突变体的能力来快速检测淋病奈瑟菌的方法已被描述。除了使用分离培养物外，转化脱氧核糖核酸还可直接从附着在宫颈或尿道拭子上的物质中获得。拭子转移至诊断实验室的时间间隔不是一个重要因素。在初次分离平板上无需使用纯培养物即可获得明确结果。在标准化条件下使用其中一种突变菌株时，非淋病奈瑟菌以及多种常见的无关细菌在转化试验中不发生反应或干扰。整个检测可在不到24小时内完成。还表明，本研究中使用的淋病奈瑟菌菌株的T4型细胞具有遗传转化能力，尽管T4型细胞的转化频率明显低于同一菌株的T2型细胞。
