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用于弯曲杆菌属的改良生长培养基。

Improved growth medium for Campylobacter species.

作者信息

Mehlman I J, Romero A

出版信息

Appl Environ Microbiol. 1982 Mar;43(3):615-8. doi: 10.1128/aem.43.3.615-618.1982.

DOI:10.1128/aem.43.3.615-618.1982
PMID:7073276
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241883/
Abstract

Campylobacter species were grown in a base containing proteose peptone no. 3, yeast extract, K2HPO4, (NH4)2SO4, NA2SO3, soluble starch, and agar. Concentrations and sources of organic nitrogen and growth factors were critical, and the optimal pH range was 7.0 to 7.5. Cultures tolerated 0.7% NaCl in addition to the salt present in the organic constituents and were sensitive to surface-active agents at concentrations recommended for enrichment of other gram-negative bacteria. Cultures were maintained on the proposed medium for 1 year with transfer every 2 weeks.

摘要

弯曲杆菌属细菌在一种含有3号蛋白胨、酵母提取物、磷酸氢二钾、硫酸铵、亚硫酸钠、可溶性淀粉和琼脂的培养基中培养。有机氮和生长因子的浓度及来源至关重要,最佳pH范围为7.0至7.5。除了有机成分中所含的盐分之外,培养物能耐受0.7%的氯化钠,并且对用于其他革兰氏阴性菌富集推荐浓度的表面活性剂敏感。培养物在该推荐培养基上每两周转接一次,可保存1年。

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本文引用的文献

1
'1001' Campylobacters: cultural characteristics of intestinal campylobacters from man and animals.“1001” 弯曲杆菌:人和动物肠道弯曲杆菌的培养特性
J Hyg (Lond). 1980 Dec;85(3):427-42. doi: 10.1017/s0022172400063506.
2
Campylobacter enteritis associated with consumption of unpasteurised milk.与饮用未巴氏杀菌牛奶相关的弯曲杆菌肠炎
Br Med J. 1979 May 5;1(6172):1171-3. doi: 10.1136/bmj.1.6172.1171.
3
The genus Campylobacter.弯曲杆菌属。
Annu Rev Microbiol. 1978;32:673-709. doi: 10.1146/annurev.mi.32.100178.003325.