Centre for Energy Research, Institute of Technical Physics and Materials Science, Eötvös Loránd Research Network, Konkoly Thege Miklós Str. 29-33, H-1121 Budapest, Hungary.
77 Elektronika Ltd., Fehérvári Str. 98, H-1111 Budapest, Hungary.
Sensors (Basel). 2023 Jan 10;23(2):800. doi: 10.3390/s23020800.
The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (Ø = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 µm) at different flow rates (0.5, 1, 2 µL/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped (), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, , and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel.
这项研究的目的是更好地解释Dean 涡旋如何影响横向聚焦，并了解与球形颗粒相比，细胞形态如何改变聚焦位置。使用不同粒径（Ø = 0.5、1.1、1.97、2.9、4.8、5.4、6.08、10.2、15.8、16.5 µm）的聚苯乙烯荧光珠，在不同流速（0.5、1、2 µL/s）下，研究了聚焦区域的位置和范围。尺寸依赖性聚焦生成了球形珠在周期性变化通道末端的平衡位置的精确图谱，为聚焦多维颗粒和细胞提供了良好的基准。实验中使用的生物样本为棒状的（）、双凹盘状的红细胞（RBC）、圆形或椭圆形的酵母、、以及柔软不规则形状的 HeLa 癌细胞系细胞，以了解细胞形状如何影响通道末端的聚焦位置。
