Pinheiro Guilherme Garcia, Rocha Marcele Neves, de Oliveira Maria Angélica, Moreira Luciano Andrade, Andrade Filho José Dilermando
Coleção de Mosquitos Neotropicais, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, Brazil.
Grupo de Estudos em Leishmanioses, Instituto René Rachou, Avenida Augusto de Lima, 1715, Belo Horizonte 30190-002, Brazil.
Insects. 2019 May 10;10(5):136. doi: 10.3390/insects10050136.
Brazil has experienced several arbovirus outbreaks in recent years, among which yellow fever stands out. The state of Minas Gerais faced outbreaks of sylvatic yellow fever in 2017 and 2018, with 1002 confirmed cases and 340 deaths. This work presents the results of survey efforts to detect the yellow fever virus in mosquitoes from two conservation areas in the metropolitan region of Belo Horizonte, Brazil. A total of 867 mosquitoes of 20 species were collected between September 2017 and May 2018, the most abundant being () (von Humboldt, 1819) (31.3%), Theobald, 1901 (19.1%) and () Dyar, 1921 (18.2%). Total RNA was extracted from the mosquitoes for real-time PCR analysis for yellow fever, chikungunya, mayaro, Zika and dengue viruses. The yellow fever infection rate was 8.2% for (13 mosquitoes), which is the main vector of sylvatic yellow fever in Brazil. In addition to surveying the mosquito fauna of these conservation units, this work demonstrates the importance of monitoring the circulation of viruses near large urban centers.
近年来，巴西经历了几次虫媒病毒疫情，其中黄热病尤为突出。米纳斯吉拉斯州在2017年和2018年面临丛林型黄热病疫情，有1002例确诊病例和340人死亡。这项工作展示了在巴西贝洛奥里藏特大都市区两个保护区的蚊子中检测黄热病毒的调查结果。在2017年9月至2018年5月期间，共收集了20种867只蚊子，数量最多的是（）（冯·洪堡，1819年）（31.3%）、西奥博尔德，1901年（19.1%）和（）戴尔，1921年（18.2%）。从蚊子中提取总RNA，用于对黄热病毒、基孔肯雅病毒、马亚罗病毒、寨卡病毒和登革病毒进行实时PCR分析。（）（13只蚊子）的黄热病毒感染率为8.2%，（）是巴西丛林型黄热病的主要传播媒介。除了调查这些保护区的蚊虫种类外，这项工作还证明了监测大城市中心附近病毒传播情况的重要性。
