Klein C E, Cordon-Cardo C, Soehnchen R, Cote R J, Oettgen H F, Eisinger M, Old L J
Memorial Sloan-Kettering Cancer Center, New York, New York.
J Invest Dermatol. 1987 Nov;89(5):500-6. doi: 10.1111/1523-1747.ep12460996.
Six cell surface glycoproteins defined by monoclonal antibodies were selected for study on human epidermal cells. In tests on tissue sections, three of the glycoproteins [J143 (gp140/30); T43 (gp85/36); H99 (gp38)] were expressed in the basal cell layer of the epidermis, whereas the other three glycoproteins [T179 (gp140/95); T16 (gp40/50); BT15 (gp80)] were preferentially expressed in maturing keratinocytes above the basal layer. We compared synthesis of these glycoproteins in fresh epidermis and in primary epidermal short term cultures using [35S]methionine for metabolic labeling. Synthesis of J143 was 8- to 20-fold higher and synthesis of T43 was 4- to 10-fold lower in cultured cells compared with fresh epidermis. BT15, an antigen strongly expressed on terminally differentiating keratinocytes, was synthesized at 5- to 15-fold higher levels in fresh epidermis than in cultured cells. Biosynthesis levels of H99, T179, and T16 did not change in cultured epidermal cells. Based on our findings, we propose a model of surface antigenic changes that occur during keratinocyte differentiation in vivo.
选择了六种由单克隆抗体定义的细胞表面糖蛋白，用于对人表皮细胞进行研究。在组织切片检测中，其中三种糖蛋白[J143（gp140/30）；T43（gp85/36）；H99（gp38）]在表皮的基底层表达，而另外三种糖蛋白[T179（gp140/95）；T16（gp40/50）；BT15（gp80）]则优先在基底层上方成熟的角质形成细胞中表达。我们使用[35S]甲硫氨酸进行代谢标记，比较了新鲜表皮和原代表皮短期培养物中这些糖蛋白的合成情况。与新鲜表皮相比，培养细胞中J143的合成高出8至20倍，T43的合成低4至10倍。BT15是一种在终末分化角质形成细胞上强烈表达的抗原，在新鲜表皮中的合成水平比培养细胞高5至15倍。培养的表皮细胞中H99、T179和T16的生物合成水平没有变化。基于我们的研究结果，我们提出了一种体内角质形成细胞分化过程中发生的表面抗原变化模型。
