Dėnė Lina, Laužikė Kristina, Rasiukevičiūtė Neringa, Chrapačienė Simona, Brazaitytė Aušra, Viršilė Akvilė, Vaštakaitė-Kairienė Viktorija, Miliauskienė Jurga, Sutulienė Rūta, Samuolienė Giedrė, Valiuškaitė Alma
Laboratory of Plant Protection, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Babtai, Lithuania.
Laboratory of Plant Physiology, Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Babtai, Lithuania.
Front Plant Sci. 2023 Jan 5;13:1098048. doi: 10.3389/fpls.2022.1098048. eCollection 2022.
Essential oils and extracts are investigated in sustainable plant protection area lately. Alternative antifungal substances are especially relevant for major economic-relevance pathogens, like (causal agent of strawberry grey mold), control. However, the reaction of plants to alternative protection with plant-origin products is currently unknown. Induced stress in plants causes changes in antioxidant and photosynthetic systems. The aim of the research was to determine the defense response of strawberry plants under application of coriander seed products. In the first step of the research, we determined coriander seed (), black seed (Nigella sativa) and peppermint leaf ( × ) products' antifungal activity against . Secondly, we continued evaluation of antifungal activity under controlled environment on strawberry plants of the most effective coriander seed products. Additionally, we evaluated the antioxidant and photosynthetic parameters in strawberries, to examine the response of plants. Antifungal activity on strawberries was determined based on grey mold incidence and severity after application of coriander products. Impact on photosynthetic system was examined measuring photosynthetic rate, transpiration rate, stomatal conductance, and intercellular to ambient CO concentration. Strawberry leaves were collected at the end of the experiment to analyze the antioxidant response. The highest antifungal activity both and on strawberries had coriander seed essential oil, which decreased grey mold severity. Coriander extract increased the photosynthetic capacity and antioxidant response of strawberry plants, however had negative effect on suppression of grey mold. In most cases, the essential oil activated antioxidant response of strawberry plants lower than extract. Our study results provide no direct impact of increased photosynthetic capacity values and antifungal effect after treatment with natural oils. The highest concentrations of coriander essential oil and extract potentially demonstrated a phytotoxic effect.
近年来，精油和提取物在可持续植物保护领域得到了研究。替代抗真菌物质对于具有重大经济意义的病原体，如草莓灰霉病（草莓灰霉病的病原体）的防治尤为重要。然而，目前尚不清楚植物对植物源产品替代保护的反应。植物中的诱导胁迫会导致抗氧化和光合系统发生变化。本研究的目的是确定在施用芫荽籽产品的情况下草莓植株的防御反应。在研究的第一步，我们测定了芫荽籽、黑种草籽和薄荷叶产品对草莓灰霉病菌的抗真菌活性。其次，我们在可控环境下继续评估最有效的芫荽籽产品对草莓植株的抗真菌活性。此外，我们还评估了草莓中的抗氧化和光合参数，以检测植株的反应。根据施用芫荽产品后草莓灰霉病的发病率和严重程度来确定其对草莓的抗真菌活性。通过测量光合速率、蒸腾速率、气孔导度和细胞间与环境CO₂浓度来检测对光合系统的影响。在实验结束时采集草莓叶片以分析抗氧化反应。芫荽籽精油对草莓灰霉病菌和草莓的抗真菌活性最高，降低了灰霉病的严重程度。芫荽提取物提高了草莓植株的光合能力和抗氧化反应，但对灰霉病的抑制有负面影响。在大多数情况下，精油激活草莓植株抗氧化反应的程度低于提取物。我们的研究结果表明，用天然油处理后光合能力值的增加和抗真菌效果没有直接影响。芫荽精油和提取物的最高浓度可能表现出植物毒性作用。
