Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Biomed Res Int. 2023 Jan 12;2023:4868048. doi: 10.1155/2023/4868048. eCollection 2023.
Stem cells provided new opportunity to treat various diseases, including liver disorders. Stem cells are unspecialized cells, stimulating influential research interest be indebted to their multipotent self-renewal capacity and differentiation characteristics into several specialized cell types. Many factors contribute to their differentiation into different cell types such as insulin producing cells, osteoblast, and hepatocytes. Accordingly, wide range methods and materials have been used to transform stem cells into hepatocytes, but effectiveness of differentiation is different and depends on several factors such as cell-to-cell adhesion, cell-to-cell contact, and cell biological change. Search was done in PubMed, Scopus, and WOS to evaluate results of studies about stem cells differentiation for higher efficacy. Among more than 28000 papers, 51 studies were considered eligible for more evaluations. Results indicated that most studies were performed on mesenchymal stem cells compared with other types. Acute liver failure was the most investigated liver disorder, and tissue engineering was the most investigated differentiation methods. Also, functional parameters were the most evaluated parameters in assessing differentiation efficacy. We summarize recent advances in increasing efficiency of stem cells differentiation using varied materials, since promising results of this review, further studies are needed to assess efficiency and safety of these cells transplantation in some liver disease treatment.
干细胞为治疗各种疾病，包括肝脏疾病，提供了新的机会。干细胞是未分化的细胞，由于其多能性自我更新能力和分化为几种特化细胞类型的特征，激发了有影响力的研究兴趣。许多因素有助于它们分化为不同的细胞类型，如产生胰岛素的细胞、成骨细胞和肝细胞。因此，已经使用了广泛的方法和材料来将干细胞转化为肝细胞，但分化的有效性是不同的，并且取决于几个因素，如细胞间粘附、细胞间接触和细胞生物学变化。在 PubMed、Scopus 和 WOS 中进行了搜索，以评估关于干细胞分化以提高功效的研究结果。在超过 28000 篇论文中，有 51 项研究被认为符合进一步评估的条件。结果表明，与其他类型相比，大多数研究都是在间充质干细胞上进行的。急性肝衰竭是研究最多的肝脏疾病，组织工程是研究最多的分化方法。此外，在评估分化效果时，功能参数是评估的最主要参数。我们总结了使用不同材料提高干细胞分化效率的最新进展，鉴于本综述的有希望的结果，需要进一步研究来评估这些细胞在某些肝脏疾病治疗中的移植效率和安全性。
