Brzychcy Karolina, Dróżdż Izabela, Skoczylas Sebastian, Płoszaj Tomasz, Sobolewska-Sztychny Dorota, Skibińska Małgorzata, Narbutt Joanna, Lesiak Aleksandra
Students Scientific Group at the Department of Dermatology, Paediatric Dermatology and Oncology, Medical University of Lodz, Lodz, Poland.
Department of Clinical Genetics, Medical University of Lodz, Lodz, Poland.
Postepy Dermatol Alergol. 2022 Dec;39(6):1162-1170. doi: 10.5114/ada.2022.120453. Epub 2022 Dec 22.
In the past few years, the advancement of 16S rRNA metagenomic analysis sequencing has enabled assessing the impact of gut microbiota on the development of skin disease. Alopecia areata (AA) is a nonscarring hair loss disorder with an unknown etiopathogenesis, however, it is hypothesised that a combination of genetic and environmental factors might be involved. Although numerous studies have shown that the microbiome plays a key role at the beginning of skin diseases, the link between AA and gut dysbiosis remains unclear.
To analyse the intestinal microbiome in patients suffering from AA.
The study describes the conceivable involvement of gut microbiota in the unclear pathogenesis of AA. We enrolled 25 patients, over 18 years of age with an active state of AA who donated their stool samples. The samples were examined at the human gut microbial community at the species level by metataxonomic analysis of the full-length 16S V3-V4 sequencing.
The four major genera that constitute the microbiome's core are Lachnoclostridium, Bifidobacterium, Streptococcus, and Eubacterium, as well as three major phyla: Firmicutes, Proteobacteria, and Actinobacteria. Firmicutes and Proteobacteria are overrepresented in the microflora, which might suggest a disturbed microflora. Furthermore, the composition of bacterial communities suggests a loss of overall richness and a decrease in taxonomic diversity across all samples.
This study describes, for the first time, the characteristics of the gut microbiome in AA patients and may provide new insight into the gut microbiome that may play a role in the development of AA.
在过去几年中，16S rRNA宏基因组分析测序技术的进步使得评估肠道微生物群对皮肤病发展的影响成为可能。斑秃（AA）是一种非瘢痕性脱发疾病，其病因尚不清楚，然而，据推测可能涉及遗传和环境因素的综合作用。尽管众多研究表明微生物群在皮肤病发病初期起关键作用，但AA与肠道微生物失调之间的联系仍不明确。
分析斑秃患者的肠道微生物群。
本研究描述了肠道微生物群在AA不明发病机制中可能的作用。我们招募了25名18岁以上处于AA活动期的患者，他们捐献了粪便样本。通过对全长16S V3-V4测序进行元分类分析，在物种水平上检测这些样本中的人类肠道微生物群落。
构成微生物群核心的四个主要属是瘤胃球菌属、双歧杆菌属、链球菌属和真杆菌属，以及三个主要门：厚壁菌门、变形菌门和放线菌门。厚壁菌门和变形菌门在微生物区系中占比过高，这可能表明微生物区系紊乱。此外，细菌群落的组成表明所有样本的总体丰富度下降，分类多样性降低。
本研究首次描述了斑秃患者肠道微生物群的特征，并可能为可能在斑秃发展中起作用的肠道微生物群提供新的见解。
