Genotypic and phenotypic tetracycline-based properties of isolates from bovine samples.

The purpose of this study was to investigate the tetracycline resistance in isolates from bovine samples in Burdur, Turkiye, and assess 16 tetracycline-resistance genes distribution among the isolates. Forty-nine isolates were phenotypically characterized for anti-microbial resistance to doxycycline, oxytetracycline and tetracycline by disc diffusion method. Presence of tetracycline genes of was investigated by multiplex and singleplex polymerase chain reaction. Our results indicated that 87.80% and 42.86% of the isolates were resistant to tetracycline and oxytetracycline, respectively, and the rate of resistance to doxycycline was 6.12%. Total of 21 (42.85%) were carrying tetracycline-resistance genes and (A) was present in 12 (24.49%) isolates; whereas, the (W) gene was identified in 9 (18.37%) and 2 (4.08%) of the isolates carried both (A) and (W), respectively. The study indicated antibiotic resistance patterns of tetracycline agents and links to the tet-genes among were detected. It makes it worthwhile that this is the first report for detection of (A) gene in .