Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Appl Biochem Biotechnol. 2023 Jul;195(7):4308-4320. doi: 10.1007/s12010-023-04316-0. Epub 2023 Jan 23.
The fabrication of multifunctional scaffolds has attracted much attention in biological fields. In this research, some novel composites of Cu(II) or Zn(II) metal-organic framework (M-MOF) and polycaprolactone (PCL), M-MOF@PCL, have been fabricated as multifunctional scaffolds for application in the tissue engineering (TE) field. The porous three-dimensional sponges were prepared by the salt leaching method. Then, the M-MOF@PCL composite sponges have been prepared by in situ synthesis of M-MOF in the presence of the as-obtained PCL sponge to gain a new compound with proper features for biological applications. Finally, curcumin was attached to the M-MOF@PCL as a bioactive compound that can act as a wound-healing agent, anti-oxidant, and anti-inflammatory. The presence of the M-MOF in final composites was investigated by different methods such as FTIR (Fourier-transform infrared), XRD (X-ray diffraction), SEM (scanning electron microscope), EDS (energy-dispersive X-ray spectroscopy), and TEM (transmission electron microscope). SEM images confirmed the porous structure of the as-obtained composites. According to the EDS and TEM images, M-MOFs were uniformly incorporated throughout the PCL sponges. The water sorption capacities of the blank PCL, Cu-MOF@PCL, and Zn-MOF@PCL were determined as 56%, 155%, and 119%, respectively. In vivo investigation on a third-degree burn model in adult male Wistar rats exhibited an accelerated wound healing for Cu-MOF@PCL compared to with Zn-MOF@PCL and the control group.
多功能支架的制备在生物领域引起了广泛关注。在这项研究中，我们制备了一些新型的铜（II）或锌（II）金属有机骨架（M-MOF）和聚己内酯（PCL）的复合材料，即 M-MOF@PCL，作为多功能支架应用于组织工程（TE）领域。通过盐溶法制备了多孔三维海绵。然后，通过在获得的 PCL 海绵存在下原位合成 M-MOF，制备了 M-MOF@PCL 复合海绵，获得了一种具有适当生物应用特性的新化合物。最后，将姜黄素附着到 M-MOF@PCL 上，作为一种具有伤口愈合、抗氧化和抗炎作用的生物活性化合物。通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）、能谱（EDS）和透射电子显微镜（TEM）等不同方法研究了最终复合材料中 M-MOF 的存在。SEM 图像证实了所获得复合材料的多孔结构。根据 EDS 和 TEM 图像，M-MOF 均匀地分布在 PCL 海绵中。空白 PCL、Cu-MOF@PCL 和 Zn-MOF@PCL 的吸水率分别为 56%、155%和 119%。在成年雄性 Wistar 大鼠三度烧伤模型中的体内研究表明，与 Zn-MOF@PCL 和对照组相比，Cu-MOF@PCL 具有加速的伤口愈合作用。
