Sauer M V, Rodi I A, Simon J A, Buster J E
Division of Reproductive Endocrinology and Infertility, University of California at Los Angeles School of Medicine, Harbor-UCLA Medical Center, Torrance 90509.
J Reprod Med. 1987 Sep;32(9):688-92.
We prepared a group of five agonadal women to receive donated embryos by delivering physiologic concentrations of 17 beta-estradiol (E2) and progesterone (P4) through a polysiloxane vaginal ring and cylinder delivery system. In order to detect endometrial surface ultrastructural asynchrony that might reduce prospects for successful embryo transfer, we performed scanning electron microscopy (SEM) on biopsies obtained from these five women and compared them against those from four normal, spontaneously ovulating controls. Light microscopy revealed all nine women to be in the late luteal phase (day 26 +/- 2) and with glandular-stromal synchrony. Further concordance was seen with SEM, with no discernible difference in the development of cilia, microcilia or apical secretory protrusions between the two groups. SEM, a technique that permits visual assessment of endometrial surface topography, demonstrated that this regimen of native E2 and P4 restored the surface ultrastructure of the prematurely menopausal human endometrium to correspond to that in the normal menstrual cycle.
我们准备了一组五名无性腺女性，通过聚硅氧烷阴道环和柱状给药系统给予生理浓度的17β-雌二醇（E2）和孕酮（P4），以接受捐赠胚胎。为了检测可能降低胚胎移植成功率的子宫内膜表面超微结构不同步情况，我们对这五名女性获取的活检组织进行了扫描电子显微镜（SEM）检查，并将其与四名正常自发排卵对照者的活检组织进行比较。光学显微镜检查显示，所有九名女性均处于黄体晚期（第26±2天），且腺-基质同步。SEM检查进一步证实了这一点，两组之间纤毛、微纤毛或顶端分泌突起的发育没有明显差异。SEM是一种能够直观评估子宫内膜表面形态的技术，它表明这种天然E2和P4方案可使过早绝经的人类子宫内膜表面超微结构恢复正常月经周期中的状态。
