Department of Microbiology and Oral Infection, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University, Yonago, Tottori, Japan.
Commun Biol. 2023 Jan 23;6(1):94. doi: 10.1038/s42003-023-04472-3.
Many bacteria belonging to the phylum Bacteroidetes move on solid surfaces, called gliding motility. In our previous study with the Bacteroidetes gliding bacterium Flavobacterium johnsoniae, we proposed a helical loop track model, where adhesive SprB filaments are propelled along a helical loop on the cell surface. In this study, we observed the gliding cell rotating counterclockwise about its axis when viewed from the rear to the advancing direction of the cell and revealed that one labeled SprB focus sometimes overtook and passed another SprB focus that was moving in the same direction. Several electron microscopic analyses revealed the presence of a possible multi-rail structure underneath the outer membrane, which was associated with SprB filaments and contained GldJ protein. These results provide insights into the mechanism of Bacteroidetes gliding motility, in which the SprB filaments are propelled along tracks that may form a multi-rail system underneath the outer membrane. The insights may give clues as to how the SprB filaments get their driving force.
许多属于拟杆菌门的细菌在固体表面上移动，称为滑行运动。在我们之前对滑行细菌黄杆菌的研究中，我们提出了一个螺旋环轨模型，其中粘性的 SprB 丝沿着细胞表面的螺旋环移动。在这项研究中，我们观察到从细胞的后端到前进方向观察时，滑行细胞逆时针旋转，并且发现一个标记的 SprB 焦点有时会超过并超过另一个以相同方向移动的 SprB 焦点。几项电子显微镜分析揭示了外膜下可能存在多轨结构，该结构与 SprB 丝有关，并包含 GldJ 蛋白。这些结果为拟杆菌滑行运动的机制提供了一些见解，在该机制中，SprB 丝沿着可能在外膜下形成多轨系统的轨道移动。这些见解可能为 SprB 丝获得驱动力的方式提供线索。
