Vinmec Research Institute of Stem Cell and Gene Technology, Vinmec Healthcare System, 458 Minh Khai, Hanoi, Vietnam.
University of Science, Vietnam National University, 334 Nguyen Trai, Hanoi, Vietnam.
BMC Neurol. 2023 Jan 23;23(1):37. doi: 10.1186/s12883-023-03071-3.
This study aimed to identify the density of mononuclear cells (MNCs) and CD34+ cells in the bone marrow of patients with three neurologic conditions.
The study included 88 patients with three neurologic conditions: 40 with cerebral palsy (CP) due to oxygen deprivation (OD), 23 with CP related to neonatal icterus (NI), and 25 with neurological sequelae after traumatic brain injury. Bone marrow aspiration was conducted from the patients' bilateral anterior iliac crest under general anesthesia in an operating theater. MNCs were isolated by Ficoll gradient centrifugation and then infused intrathecally.
There was a significant difference in the average MNC per ml and percentage of CD34+ cells by the type of disease, age group, and infusion time (p value < 0.05). The multivariable regression model showed the percentage of CD34+ association with the outcome (gross motor function 88 items- GMFM-88) in patients with CP.
The density of MNCs was 5.22 million cells per mL and 5.03% CD34+ cells in patients with three neurologic conditions. The highest density of MNCs in each ml of bone marrow was found in patients with CP due to OD, whereas the percentage of CD34+ cells was the highest among patients with CP related to NI.
本研究旨在确定三种神经疾病患者骨髓中单核细胞（MNC）和 CD34+细胞的密度。
研究纳入了 88 例患有三种神经疾病的患者：40 例因缺氧导致脑瘫（CP）（OD），23 例因新生儿黄疸（NI）导致 CP，25 例因创伤性脑损伤后出现神经后遗症。在手术室全身麻醉下从患者双侧髂前嵴进行骨髓抽吸。通过菲可尔梯度离心分离 MNC 并进行鞘内输注。
按疾病类型、年龄组和输注时间的不同，每毫升平均 MNC 和 CD34+细胞百分比有显著差异（p 值 < 0.05）。多变量回归模型显示，CD34+百分比与 CP 患者的结局（粗大运动功能 88 项-GMFM-88）相关。
三种神经疾病患者的 MNC 密度为每毫升 522 万个细胞和 5.03% CD34+细胞。在每毫升骨髓中 MNC 密度最高的是因 OD 导致 CP 的患者，而 CD34+细胞的百分比在因 NI 导致 CP 的患者中最高。
