Kwawukume Samuel, Velez Frank J, Williams David, Cui Leqi, Singh Prashant
Department of Nutrition, and Integrative Physiology, Florida State University, Tallahassee, FL, USA.
SeaD Consulting, 10015 Black Birch, Tomball, TX 77375, USA.
Food Chem (Oxf). 2023 Jan 16;6:100164. doi: 10.1016/j.fochms.2023.100164. eCollection 2023 Jul 30.
The Atlantic white shrimp () is of great economic importance to the United States and risk being substituted with imported species due to a shortage in domestic production. To improve the current methods used for the identification of the Atlantic white shrimp species, we designed and validated a robust multiplex PCR-lateral flow assay for the onsite identification of . The standardized assay was validated using a miniaturized, low-cost PCR instrument with 68 shrimp, prawn, and fish samples, spread over fourteen seafood species. was simultaneously amplified by the multiplex assay to give three visual bands, which distinguished it from other species having either one or two bands on the dipstick. The standardized assay showed 100% inclusivity for target samples, 100% exclusivity for non-target samples and can be completed in less than two hours. The assay standardized in this study can be used for onsite testing of samples at processing facilities, restaurants, and wholesalers' facilities.
大西洋白虾（）对美国具有重要的经济意义，由于国内产量短缺，面临被进口品种替代的风险。为改进目前用于鉴定大西洋白虾物种的方法，我们设计并验证了一种用于现场鉴定的稳健多重PCR - 侧流分析方法。使用一台小型、低成本的PCR仪器，对68份虾、对虾和鱼类样本（涵盖14种海鲜物种）进行验证，从而验证了该标准化分析方法。通过多重分析同时扩增，产生三条可见条带，将其与试纸条上有一条或两条条带的其他物种区分开来。标准化分析方法对目标样本的包容性为100%，对非目标样本的排他性为100%，且可在两小时内完成。本研究中标准化的分析方法可用于在加工设施、餐馆和批发商设施中对样本进行现场检测。
