Liu Gang, Xu Na, Feng Jiahui
School of Life Sciences, Anhui Medical University, Hefei, China.
Front Microbiol. 2023 Jan 9;13:1081468. doi: 10.3389/fmicb.2022.1081468. eCollection 2022.
Migratory birds are the primary source and reservoir of antibiotic-resistant genes (ARGs) related to their gut microbes. In this study, we performed metagenomics analysis to study the gut microbial communities and ARGs of wintering at Shengjin (SJ) and Caizi (CZ) Lakes. The results showed that bacteria, fungi, viruses, and archaea were the dominant gut microbes. Principal component analysis (PCA) indicated that the microbiota compositions significantly differed between the two populations. Diet may be the most crucial driver of the gut microbial communities for . This species fed exclusively on spp. at Shengjin Lake and primarily on spp. at Caizi Lake. Tetracycline, macrolide, fluoroquinolone, phenicol, and peptide antibiotics were the dominant resistant types. ARGs had a significantly higher abundance of operational taxonomic units (OTUs) in the Shengjin Lake samples than in Caizi Lake samples. PCA indicated that most Shengjin Lake samples significantly differed in gut microbiota composition from those obtained at Caizi Lake. This difference in gut microbiota composition between the two lakes' samples is attributed to more extensive aquaculture operations and poultry farms surrounding Shengjin Lake than Caizi Lake. ARGs-microbes associations indicated that 24 bacterial species, commonly used as indicators of antibiotic resistance in surveillance efforts, were abundant in wintering . The results revealed the composition and structural characteristics of the gut microbiota and ARGs of , pointing to their high sensitivities to diet habits at both lakes. This study also provides primary data for risk prevention and control of potential harmful pathogens that could endanger public health and therefore are of major significance to epidemiological and public health.
候鸟是与其肠道微生物相关的抗生素抗性基因（ARGs）的主要来源和储存库。在本研究中，我们进行了宏基因组学分析，以研究在升金湖（SJ）和菜子湖（CZ）越冬的[某种候鸟]的肠道微生物群落和ARGs。结果表明，细菌、真菌、病毒和古菌是主要的肠道微生物。主成分分析（PCA）表明，两个种群的微生物群组成存在显著差异。饮食可能是[某种候鸟]肠道微生物群落的最关键驱动因素。该物种在升金湖仅以[某种食物]为食，而在菜子湖主要以[另一种食物]为食。四环素、大环内酯类、氟喹诺酮类、氯霉素类和肽类抗生素是主要的抗性类型。升金湖样本中ARGs的操作分类单元（OTUs）丰度显著高于菜子湖样本。PCA表明，大多数升金湖样本的肠道微生物群组成与在菜子湖获得的样本有显著差异。两个湖泊样本之间肠道微生物群组成的这种差异归因于升金湖周围比菜子湖有更广泛的水产养殖作业和家禽养殖场。ARGs-微生物关联表明，24种细菌物种，在监测工作中常用作抗生素抗性指标，在越冬的[某种候鸟]中大量存在。结果揭示了[某种候鸟]肠道微生物群和ARGs的组成及结构特征，表明它们对两个湖泊的饮食习惯高度敏感。本研究还为可能危害公众健康的潜在有害病原体的风险预防和控制提供了基础数据，因此对流行病学和公共卫生具有重要意义。
