Grotendorst G R
Methods Enzymol. 1987;147:144-52. doi: 10.1016/0076-6879(87)47105-x.
The method described here for the quantitation of cell migration in Boyden chambers is applicable to many cell types, especially connective tissue cells and endothelial cells. Currently, there are many groups working toward the isolation of chemotactic factors for these cells and this method would be useful for the large numbers of repetitive assays done during a purification. In our hands the assay is very reproducible and can be learned within a short time. The major problem with the assay is that the upper surface cells must be completely removed as well as any cellular debris containing dye. However, if the proper care is used in removing these cells, the results vary less than those obtained by counting cells directly.
此处描述的用于定量博伊登小室中细胞迁移的方法适用于多种细胞类型，尤其是结缔组织细胞和内皮细胞。目前，有许多研究小组致力于分离这些细胞的趋化因子，该方法对于纯化过程中进行的大量重复性检测将非常有用。在我们的操作中，该检测具有很高的可重复性，并且可以在短时间内学会。该检测的主要问题是必须完全去除上表面的细胞以及任何含有染料的细胞碎片。然而，如果在去除这些细胞时小心操作，结果的差异要小于直接计数细胞所获得的结果。
