铜离子通过激活变形链球菌活性氮物种抑制变形链球菌-小韦荣球菌双重生物膜。
Copper ions inhibit Streptococcus mutans-Veillonella parvula dual biofilm by activating Streptococcus mutans reactive nitrogen species.
机构信息
State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
出版信息
BMC Oral Health. 2023 Jan 28;23(1):48. doi: 10.1186/s12903-023-02738-0.
BACKGROUND
To investigate the inhibition mechanism of copper ions on Streptococcus mutans-Veillonella parvula dual biofilm.
METHODS
S. mutans-V. parvula dual biofilm was constructed and copper ions were added at different concentrations. After the biofilm was collected, RNA-seq and qRT-PCR were then performed to get gene information.
RESULTS
The coculture of S. mutans and V. parvula formed a significantly better dual biofilm of larger biomass than S. mutans mono biofilm. And copper ions showed a more significant inhibitory effect on S. mutans-V. parvula dual biofilm than on S. mutans mono biofilm when copper ions concentration reached 100 µM, and copper ions showed a decreased inhibitory effect on S. gordonii-V. parvula dual biofilm and S. sanguis-V.parvula dual biofilm than on the two mono biofilms as the concentration of copper ions increased. And common trace elements such as iron, magnesium, and zinc showed no inhibitory effect difference on S. mutans-V. parvula dual biofilm. The RNA-seq results showed a significant difference in the expression of a new ABC transporter SMU_651c, SMU_652c, SMU_653c, and S. mutans copper chaperone copYAZ. SMU_651c, SMU_652c, and SMU_653c were predicted to function as nitrite/nitrate transporter-related proteins, which suggested the specific inhibition of copper ions on S. mutans-V. parvula dual biofilm may be caused by the activation of S. mutans reactive nitrogen species.
CONCLUSIONS
Streptococcus mutans and Veillonella parvula are symbiotic, forming a dual biofilm of larger biomass to better resist the external antibacterial substances, which may increase the virulence of S. mutans. While common trace elements such as iron, magnesium, and zinc showed no specific inhibitory effect on S. mutans-V. parvula dual biofilm, copper ion had a unique inhibitory effect on S. mutans-V. parvula dual biofilm which may be caused by activating S. mutans RNS when copper ions concentration reached 250 µM.
背景
研究铜离子对变形链球菌-小韦荣球菌双生物膜的抑制机制。
方法
构建变形链球菌-小韦荣球菌双生物膜，加入不同浓度的铜离子。收集生物膜后，进行 RNA-seq 和 qRT-PCR 以获取基因信息。
结果
变形链球菌和小韦荣球菌共培养形成的双生物膜生物量明显大于变形链球菌单生物膜，且铜离子浓度达到 100µM 时，铜离子对变形链球菌-小韦荣球菌双生物膜的抑制作用明显大于变形链球菌单生物膜，随着铜离子浓度的增加，铜离子对血链球菌-小韦荣球菌双生物膜和血链球菌-小韦荣球菌双生物膜的抑制作用低于两种单生物膜。常见的痕量元素如铁、镁和锌对变形链球菌-小韦荣球菌双生物膜无抑制作用差异。RNA-seq 结果显示，新的 ABC 转运蛋白 SMU_651c、SMU_652c、SMU_653c 和变形链球菌铜伴侣 copYAZ 的表达存在显著差异。SMU_651c、SMU_652c 和 SMU_653c 被预测为亚硝酸盐/硝酸盐转运蛋白相关蛋白，这表明铜离子对变形链球菌-小韦荣球菌双生物膜的特异性抑制可能是由变形链球菌活性氮物质的激活引起的。
结论
变形链球菌和小韦荣球菌共生，形成更大生物量的双生物膜，以更好地抵抗外部抗菌物质，这可能增加变形链球菌的毒力。虽然常见的痕量元素如铁、镁和锌对变形链球菌-小韦荣球菌双生物膜没有特定的抑制作用，但铜离子对变形链球菌-小韦荣球菌双生物膜具有独特的抑制作用，当铜离子浓度达到 250µM 时，可能是由于激活了变形链球菌的 RNS。
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