施用 和 制剂对胡椒生长的促进作用和对 的生防作用。
Pepper growth promotion and biocontrol against by and formulations.
机构信息
Facultad de Ciencias Agrotecnológicas, Universidad Autónoma de Chihuahua, Chihuahua, México.
Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México.
出版信息
PeerJ. 2023 Jan 24;11:e14633. doi: 10.7717/peerj.14633. eCollection 2023.
BACKGROUND
genus has been used in horticultural crops as a biocontrol agent against insect pests, microbial phytopathogens, and plant growth-promoting bacteria (PGPB), representing an alternative to agrochemicals. In particular, (Bc) and (Bt) have been studied for their fungicidal and insecticidal activities. However, their use as biofertilizer formulations and biocontrol agents against phytopathogenic bacteria is limited.
OBJECTIVE
To evaluate Bc and Bt formulations as PGPB and biocontrol agents against the bacterial spot agent (Xe) in greenhouse-grown chili peppers.
METHODS
Bc and Bt isolates obtained from soil samples were identified and characterized using conventional biochemical and multiplex PCR identification methods. Bioassays to determine Bc and Bt isolates potential as PGPB were evaluated on chili pepper seedlings in seedbeds. In addition, formulations based on Bc (F-BC26 and F-BC08) and Bt (F-BT24) strains were assessed as biofertilizers on pepper, under controlled conditions. Furthermore, antagonism assays were performed by confronting Bc and Bt isolate formulations against Xe isolates in direct (foliage) and indirect (resistance induction) phytopathogen biocontrol assays on pepper plants, which were grown under controlled conditions for 15 d after formulations treatment.
RESULTS
Isolates were identified as Bc and Bt. Formulations significantly improved pepper growth in seedbeds and pots, whereas bioassays demonstrated the bactericidal effect of Bc and Bt strains against Xe isolates. Furthermore, assays showed significant plant protection by F-BC26, F-BC08, and F-BT24 formulated strains against Xe.
CONCLUSION
Results indicated that F-BT24 and F-BC26 isolates formulations promoted pepper growth and protected it against .
背景
属已被用作园艺作物的生物防治剂，以防治昆虫、微生物植物病原体和植物促生细菌 (PGPB)，代表了对农用化学品的替代。特别是 （Bc）和 （Bt）因其杀菌和杀虫活性而被研究。然而，它们作为生物肥料制剂和针对植物病原菌的生物防治剂的用途有限。
目的
评估 Bc 和 Bt 制剂作为 PGPB 和生物防治剂，防治温室种植辣椒上的细菌性斑点病病原菌 （Xe）。
方法
从土壤样本中获得的 Bc 和 Bt 分离物通过常规生化和多重 PCR 鉴定方法进行鉴定和表征。在苗床上评估 Bc 和 Bt 分离物作为 PGPB 的潜力的生物测定。此外，基于 Bc（F-BC26 和 F-BC08）和 Bt（F-BT24）菌株的制剂在受控条件下评估对辣椒的生物肥料效果。此外，通过在辣椒植物上进行直接（叶片）和间接（抗性诱导）植物病原菌生物防治测定，对抗 Bc 和 Bt 分离物制剂与 Xe 分离物的拮抗作用进行了测定，这些测定是在制剂处理后 15 天在受控条件下进行的。
结果
分离物被鉴定为 Bc 和 Bt。制剂显著改善了苗床和盆中辣椒的生长，而生物测定表明 Bc 和 Bt 菌株对 Xe 分离物具有杀菌作用。此外，测定结果表明，F-BC26、F-BC08 和 F-BT24 制剂菌株对 Xe 具有显著的植物保护作用。
结论
结果表明，F-BT24 和 F-BC26 分离物制剂促进了辣椒的生长，并保护其免受 Xe 的侵害。
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