Department of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, India.
INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) Laboratory, Division of Immunology and Infectious Diseases, Council of Scientific & Industrial Research-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, India.
Front Cell Infect Microbiol. 2023 Jan 13;12:1082674. doi: 10.3389/fcimb.2022.1082674. eCollection 2022.
sp. HM71, a human gut microbe possesses metabolic machinery to catabolize antigenic gluten, hence, holds promises as microbial therapy to treat gluten-derived celiac disease. However, its efficacy, safety, and survivability in the gastrointestinal ecosystem await functional elucidation. The current study is designed to characterize sp. HM71 for its physiological, genomic, and probiotic properties. The morphological and physiological assessment indicates it as a coccus-shaped gram-positive bacterium growing optimally at 30°C in a neutral environment (pH 7.0). sp. HM71 showed continuous growth even in stressful environments (salinity up to 3% NaCl and 6% KCl), variable temperature (25°C to 35°C) and pH (5-9), antibiotics, and gastric and intestinal conditions. The sp. HM71 genome harbors diversified genetic machinery to modulate humongous metabolic potential for the host. This was substantiated by the hemolytic and CaCo-2 cell line assay which confirms its cellular adherence and biosafety. Notably, genome analysis did not identify any pathogenic islands. Probiotic characterization indicates its potential to overcome waterborne infections and digestion-related disorders. Cumulatively, sp. HM71 can be considered a probiotic strain for improving human health because of the highlighted functions.
HM71 是人肠道微生物，具有代谢抗原性谷蛋白的机制，因此有望成为治疗源于谷蛋白的乳糜泻的微生物疗法。然而，其在胃肠道生态系统中的疗效、安全性和生存能力仍有待功能阐明。本研究旨在对 HM71 进行生理、基因组和益生菌特性的表征。形态和生理评估表明，它是一种球菌状革兰氏阳性细菌，在中性环境（pH7.0）中最适生长温度为 30°C。HM71 即使在压力环境（盐度高达 3%NaCl 和 6%KCl、温度（25°C 至 35°C）和 pH（5-9）、抗生素以及胃和肠道条件下）下也能持续生长。HM71 基因组具有多样化的遗传机制，可调节宿主巨大的代谢潜力。这一点通过溶血和 CaCo-2 细胞系测定得到了证实，该测定证实了其细胞黏附和生物安全性。值得注意的是，基因组分析未发现任何致病岛。益生菌特性表明，它有潜力克服水传播感染和与消化有关的疾病。总之，由于强调了这些功能，HM71 可以被认为是一种改善人类健康的益生菌菌株。
