Rusznyák Anna, Tóth Erika M, Schumann Peter, Spröer Cathrin, Makk Judit, Szabó Gitta, Vladár Péter, Márialigeti Károly, Borsodi Andrea K
Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C H-1117 Budapest, Hungary.
DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2011 Jul;61(Pt 7):1662-1666. doi: 10.1099/ijs.0.022608-0. Epub 2010 Aug 20.
An alkalitolerant and moderately halophilic strain, designated KB23(T), characterized by optimal growth at pH 8.0-9.0 and in the presence of 5-7 % (w/v) NaCl, was isolated from a reed (Phragmites australis) periphyton sample originating from an extremely shallow, alkaline soda pond located in Hungary. Cells of strain KB23(T) were Gram-stain-positive, motile straight rods. Strain KB23(T) was facultatively anaerobic, catalase-positive, oxidase-negative and contained peptidoglycan type A4β (L-Orn-D-Asp). MK-9(H4) was the predominant isoprenoid quinone and anteiso-C(15 : 0), C(16 : 0) and anteiso-C(15 : 1) were the major cellular fatty acids. The DNA G+C content of strain KB23(T) was 74.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belongs to the genus Cellulomonas and that it is related most closely to Cellulomonas flavigena DSM 20109(T) (97.35 % similarity), Cellulomonas terrae DB5(T) (96.81 %), Cellulomonas iranensis O(T) (96.75), Cellulomonas chitinilytica X.bu-b(T) (96.60 %), Cellulomonas persica I(T) (96.53 %), Cellulomonas composti TR7-06(T) (96.45 %), Cellulomonas biazotea DSM 20112(T) (96.34 %) and Cellulomonas fimi DSM 20113(T) (96.20 %). According to these results, together with DNA-DNA hybridization and physiological data, strain KB23(T) is considered to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas phragmiteti sp. nov. is proposed. The type strain is KB23(T) ( = DSM 22512(T) = NCAIM B002303(T)).
从匈牙利一个极浅的碱性苏打湖中的芦苇（芦苇）周丛生物样本中分离出一株耐碱中度嗜盐菌株，命名为KB23(T)，其最适生长pH为8.0 - 9.0，在5 - 7%（w/v）NaCl存在的条件下生长。KB23(T)菌株的细胞革兰氏染色阳性，为运动直杆状。菌株KB23(T)为兼性厌氧，过氧化氢酶阳性，氧化酶阴性，含有A4β型肽聚糖（L-鸟氨酸-D-天冬氨酸）。MK-9(H4)是主要的类异戊二烯醌，anteiso-C(15 : 0)、C(16 : 0)和anteiso-C(15 : 1)是主要的细胞脂肪酸。KB23(T)菌株的DNA G+C含量为74.8 mol%。基于16S rRNA基因序列的系统发育分析表明，该菌株属于纤维单胞菌属，与黄纤维单胞菌DSM 20109(T)（相似性97.35%）、土栖纤维单胞菌DB5(T)（96.81%）、伊朗纤维单胞菌O(T)（96.75）、解几丁质纤维单胞菌X.bu-b(T)（96.60%）、波斯纤维单胞菌I(T)（96.53%）、堆肥纤维单胞菌TR7-06(T)（96.45%）、双氮纤维单胞菌DSM 20112(T)（96.34%）和解纤维单胞菌DSM 20113(T)（96.20%）关系最为密切。根据这些结果，结合DNA-DNA杂交和生理数据，菌株KB23(T)被认为代表纤维单胞菌属的一个新物种，为此提出名为芦苇纤维单胞菌（Cellulomonas phragmiteti sp. nov.）。模式菌株为KB23(T)（=DSM 22512(T)=NCAIM B002303(T)）。
