Laboratory of Plant Nutrition and Fertilizers, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Japan Collection of Microorganisms, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2305-2311. doi: 10.1099/ijs.0.059063-0. Epub 2014 Apr 14.
A rod-shaped, motile, facultatively anaerobic and moderately halotolerant plant-growth-promoting actinobacterial strain, designated NCCP-11(T), was isolated from paddy grains. To delineate its taxonomic position, the strain was subjected to a polyphasic characterization. Cells of strain NCCP-11(T) grew at 10-37 °C (optimum 28-32 °C), at pH 6-9 (optimum pH 7) and in 0-12% (w/v) NaCl (optimum 1-2%) in broth medium. Based on 16S rRNA gene sequence analysis, strain NCCP-11(T) showed highest similarity to the type strains of Cellulomonas hominis (98.99%) and Cellulomonas denverensis (98.09 %) and less than 97 % with other closely related taxa. The chemotaxonomic data [major menaquinone: MK-9(H4); cell-wall peptidoglycan: type A4β; major fatty acids: anteiso-C15 : 0, C16 : 0, C14 : 0 and anteiso-C17 : 0; major polar lipids: diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannosides and two unknown polar lipids] also supported the affiliation of strain NCCP-11(T) to the genus Cellulomonas. The level of DNA-DNA relatedness between strain NCCP-11(T) and the two type strains mentioned above was less than 42.7%. On the basis of DNA-DNA relatedness, physiological and biochemical characteristics and phylogenetic position, strain NCCP-11(T) can be differentiated from species of the genus Cellulomonas with validly published names and thus represents a novel species, for which the name Cellulomonas pakistanensis sp. nov. is proposed. The type strain is NCCP-11(T) ( = DSM 24792(T) = JCM 18755(T) = KCTC 19798(T)).
一株杆状、能动、兼性厌氧和中度耐盐的植物促生放线菌菌株，被命名为 NCCP-11(T)，从稻田谷物中分离得到。为了阐明其分类地位，对该菌株进行了多相特征描述。NCCP-11(T) 菌株的细胞在 10-37°C（最佳温度为 28-32°C）、pH6-9（最佳 pH 值为 7）和 0-12%（w/v）NaCl（最佳浓度为 1-2%）的肉汤培养基中生长。根据 16S rRNA 基因序列分析，NCCP-11(T) 菌株与同源性最高的模式菌株 Homo sapiens（98.99%）和 Cellulomonas denverensis（98.09%）相似，与其他密切相关的分类群相似性低于 97%。化学分类数据[主要类异戊二烯醌：MK-9（H4）；细胞壁肽聚糖：A4β 型；主要脂肪酸：anteiso-C15：0、C16：0、C14：0 和 anteiso-C17：0；主要极性脂：双磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和两种未知极性脂]也支持 NCCP-11(T) 菌株属于 Cellulomonas 属。NCCP-11(T) 菌株与上述两种模式菌株之间的 DNA-DNA 同源性水平低于 42.7%。基于 DNA-DNA 同源性、生理生化特性和系统发育地位，NCCP-11(T) 菌株可与已发表的有效名称的 Cellulomonas 属种区分开来，因此代表一个新种，建议将其命名为 Cellulomonas pakistanensis sp. nov.。其模式菌株为 NCCP-11(T)（=DSM 24792(T)=JCM 18755(T)=KCTC 19798(T)）。
