Key Laboratory of Zoonosis Research, Ministry of Education Jilin University/College of Animal Medicine, Changchun, 130062, Jilin, China.
Panjin Center for Inspection and Testing/Panjin CDC, Panjin, 124010, Liaoning, China.
Antonie Van Leeuwenhoek. 2021 May;114(5):527-538. doi: 10.1007/s10482-021-01538-2. Epub 2021 Mar 12.
A Gram-positive, smooth, sub-transparent, faint yellow,0.5-0.7 µm diameter, rod shaped aerobic or facultative aerobic strain P40-2was isolated from livestock farms in Northeast China. Strain P40-2 grew at 25-40 °C (optimum 30-38 °C), and in 0-4% (w/v) NaCl (optimum 0%) in LB medium. Based on 16S rRNA gene sequence analysis, strain P40-2 belongs to the class Cellulomonas and is most closely related to C. denverensis strain W6929, C. pakistanensis strain NCCP-11and C. hominis strain CE40.DNA-DNA hybridization rate of strain P40-2 was 29%, and the ANI with C.denverensisstrainW6929 was 85.33%. The genome is 3437431 bp long with a G + C content of 71.99%. Of the 3177 predicted genes, 3119 were protein-coding genes and 58 were RNA encoding genes. The chemotaxonomic data: menaquinone was MK-9(H), anteiso-C, C and anteiso-C were the major cellular fatty acids, and the main cell-wall amino acids were ornithine,alanine, glycine and glutamate. The cell wall peptidogly can sugars included glucose, rhamnose, galactose and mannose. The polar lipid present were DPG, PG, PE, and PIM. On the basis of DNA-DNA relatedness, phylogenetic position, complete genome sequence and physiological characteristics, strain P40-2 can be differentiated from other species of the genus Cellulomonas with validly published names and thus represents a novel species, for which the name Cellulomonas taurus is proposed. The type strain is Cellulomonas taurus P40-2 (= CGMCC No.1.17732).The acute toxicity test in mice showed that LD of strain P40-2 was rather high with 1.5 × 10 CFU/mouse, which indicated low pathogenicity. Drug susceptibility showed that strainP40-2 was resistant to most antibiotics and only sensitive to six antibiotics. Strain P40-2 contained a variety of hydrolytic enzymes including the ability to hydrolyze cellulose, β-glucan, chitin, xylan, and casein. Microbial flocculant MBF-P40 for sewage was prepared with strain P40-2, after strain P40-2 was confirmed that had good flocculation effect. MBF-P40 was used to prepare flocculation rate of 99.40%. MBF-P40 treatmented sewage from eight different sources. Flocculation rate for pig farm wastewater was 96.07%, COD removal rate is 71.05%, ammonia nitrogen removal rate is 18.22%. The result shows that MBF-P40 has a good flocculation effect, and good prospect of development and application for wastewater treatment.
一株革兰氏阳性、光滑、半透明、浅黄色、0.5-0.7µm 直径、杆状需氧或兼性需氧菌株 P40-2 从中国东北的牲畜养殖场中分离得到。P40-2 菌株在 25-40°C（最佳 30-38°C）和 0-4%（w/v）NaCl（最佳 0%）的 LB 培养基中生长。根据 16S rRNA 基因序列分析，P40-2 菌株属于纤维单胞菌属，与 C. denverensis 菌株 W6929、C. pakistanensis 菌株 NCCP-11 和 C. hominis 菌株 CE40 最为密切相关。P40-2 菌株的 DNA-DNA 杂交率为 29%，与 C. denverensis 菌株 W6929 的 ANI 为 85.33%。基因组全长 3437431bp，G+C 含量为 71.99%。在 3177 个预测基因中，有 3119 个是蛋白质编码基因，58 个是 RNA 编码基因。化学分类学数据：menaquinone 为 MK-9(H)、anteiso-C、C 和 anteiso-C 是主要的细胞脂肪酸，主要的细胞壁氨基酸是鸟氨酸、丙氨酸、甘氨酸和谷氨酸。细胞壁肽聚糖糖包括葡萄糖、鼠李糖、半乳糖和甘露糖。存在的极性脂质是 DPG、PG、PE 和 PIM。基于 DNA-DNA 相关性、系统发育位置、全基因组序列和生理特性，P40-2 菌株可与已发表的有效名称的纤维单胞菌属其他种区分开来，因此代表一个新种，提议将其命名为金牛座纤维单胞菌。模式菌株为金牛座纤维单胞菌 P40-2（=CGMCC No.1.17732）。在小鼠的急性毒性试验中，P40-2 菌株的 LD 相当高，为 1.5×10 CFU/只，表明其致病性较低。药敏试验表明，P40-2 菌株对大多数抗生素具有抗性，仅对 6 种抗生素敏感。P40-2 菌株含有多种水解酶，包括纤维素、β-葡聚糖、壳聚糖、木聚糖和酪蛋白的水解能力。用 P40-2 菌株制备了用于污水的微生物絮凝剂 MBF-P40，经确认 P40-2 菌株具有良好的絮凝效果。MBF-P40 用于制备絮凝率为 99.40%。MBF-P40 处理了来自八个不同来源的污水。猪场废水的絮凝率为 96.07%，COD 去除率为 71.05%，氨氮去除率为 18.22%。结果表明，MBF-P40 具有良好的絮凝效果，在污水处理方面具有良好的开发应用前景。
