Holman Ryan, Lorton Orane, Guillemin Pauline C, Desgranges Stéphane, Santini Francesco, Preso Davide Bernardo, Farhat Mohamed, Contino-Pépin Christiane, Salomir Rares
Image Guided Interventions Laboratory (GR-949), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Avignon Université, Equipe Systèmes Amphiphiles bioactifs et Formulations Eco-compatibles, Unité Propre de Recherche et d'Innovation (UPRI), Avignon, France.
Front Oncol. 2023 Jan 13;12:1025481. doi: 10.3389/fonc.2022.1025481. eCollection 2022.
Sonosensitive perfluorocarbon FTAC-PFOB emulsion is under development to enhance heating, increase thermal contrast, and reduce treatment times during focused ultrasound tumor ablation of highly perfused tissue. The emulsion previously showed enhanced heating during and studies. Experiments were designed to observe the response in additional scenarios by varying focused ultrasound conditions, emulsion concentrations, and surfactants. Most notably, changes in acoustic absorption were assessed with MR-ARFI. Phantoms were developed to have thermal, elastic, and relaxometry properties similar to those of pig tissue. The phantoms were embedded with varying amounts of FTAC-PFOB emulsion or lecithin-PFOB emulsion, between about 0.0-0.3% v:w, in 0.05% v:w increments. MR-ARFI measurements were performed using a FLASH-ARFI-MRT sequence to obtain simultaneous displacement and temperature measurements. A Fabry-Perot hydrophone was utilized to observe the acoustic emissions. Susceptibility-weighted imaging and relaxometry mapping were performed to observe concentration-dependent effects. F diffusion-ordered spectroscopy NMR was used to measure the diffusion coefficient of perfluorocarbon droplets in a water emulsion. Increased displacement and temperature were observed with higher emulsion concentration. In semi-rigid MR-ARFI phantoms, a linear response was observed with low-duty cycle MR-ARFI sonications and a mono-exponential saturating response was observed with sustained sonications. The emulsifiers did not have a significant effect on acoustic absorption in semi-rigid gels. Stable cavitation might also contribute to enhanced heating.
对超声敏感的全氟碳FTAC-PFOB乳剂正在研发中，用于在对高灌注组织进行聚焦超声肿瘤消融时增强加热效果、增加热对比度并缩短治疗时间。该乳剂在之前的[具体研究]和[具体研究]中已显示出增强的加热效果。实验旨在通过改变聚焦超声条件、乳剂浓度和表面活性剂来观察在其他情况下的反应。最值得注意的是，利用磁共振声辐射力成像（MR-ARFI）评估了声吸收的变化。制作了具有与猪组织相似的热、弹性和弛豫特性的体模。体模中嵌入了不同量的FTAC-PFOB乳剂或卵磷脂-PFOB乳剂，含量在约0.0-0.3%（体积比：重量比）之间，以0.05%（体积比：重量比）的增量递增。使用FLASH-ARFI-MRT序列进行MR-ARFI测量，以同时获得位移和温度测量结果。利用法布里-珀罗水听器观察声发射。进行了磁化率加权成像和弛豫测量成像，以观察浓度依赖性效应。利用F扩散排序光谱核磁共振测量水乳液中全氟碳液滴的扩散系数。随着乳剂浓度的增加，观察到位移和温度升高。在半刚性MR-ARFI体模中，低占空比MR-ARFI超声处理时观察到线性反应，持续超声处理时观察到单指数饱和反应。乳化剂对半刚性凝胶中的声吸收没有显著影响。稳定的空化也可能有助于增强加热效果。
