在多孔组织培养板中孵育期间乙醇浓度的变化。

Changes in ethanol concentration during incubation in multiwell tissue culture trays.

作者信息

Hurley M M, Martin D, Raisz L G

机构信息

Division of Endocrinology and Metabolism, University of Connecticut Health Center, Farmington 06032.

出版信息

Proc Soc Exp Biol Med. 1987 Nov;186(2):139-41. doi: 10.3181/00379727-186-42593.

DOI:10.3181/00379727-186-42593

PMID:3671353

Abstract

Ethanol can have direct effects in tissue culture and is often used as a solvent. Analysis of these effects will require a precise knowledge of the concentration over time in the particular system employed. We measured the disappearance rate of ethanol from multiwell culture trays (open system) containing single or multiple concentrations of ethanol over a 48-hr time period. The ethanol concentration was also measured at 72 hr in stoppered Erlenmeyer flasks (closed system). In multiwell culture trays, 1 and 0.3% ethanol (V/V) evaporated with a t1/2 of 6 to 12 hr. Media containing no ethanol but adjacent to wells with 1% ethanol accumulated ethanol with a peak of 0.2% at 12 hr. The evaporation of 0.3% ethanol was slower from wells adjacent to those containing 1% ethanol. At 72 hr, stoppered Erlenmeyer flasks, which originally contained 1% ethanol, still had a concentration of 0.85%. Since both evaporation and transfer can occur in an open system, it is necessary to specify precise conditions or measure concentrations in such systems. Alternatively, a closed system in which the concentration of ethanol is maintained can be employed.

摘要

乙醇在组织培养中会产生直接影响，并且常被用作溶剂。对这些影响进行分析需要精确了解所使用的特定系统中乙醇浓度随时间的变化情况。我们测量了在48小时时间段内，含有单浓度或多浓度乙醇的多孔培养板（开放系统）中乙醇的消失速率。同时，我们也测量了在封闭的锥形瓶（封闭系统）中72小时时的乙醇浓度。在多孔培养板中，1%和0.3%（V/V）的乙醇蒸发，其半衰期为6至12小时。不含乙醇但与含1%乙醇的孔相邻的培养基在12小时时积累了乙醇，峰值为0.2%。与含1%乙醇的孔相邻的孔中，0.3%乙醇的蒸发较慢。在72小时时，最初含有1%乙醇的封闭锥形瓶中，乙醇浓度仍为0.85%。由于在开放系统中蒸发和转移都可能发生，因此有必要明确精确的条件或测量此类系统中的浓度。或者，可以采用能保持乙醇浓度的封闭系统。