Rodríguez F D, Simonsson P, Alling C
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Salamanca, Spain.
Alcohol Alcohol. 1992 May;27(3):309-13.
The present study reports on the development of a model for maintaining constant ethanol concentrations over time in cell culture media. When neuroblastoma x glioma cells (NG 108-15) were grown in ethanol containing media under standard cultivation conditions in the incubator at 37 degrees C, a 90% evaporation was observed after 24 hr. To counteract evaporation, the cell culture dishes were placed inside polystyrene boxes together with an open dish containing an appropriate amount of ethanol. By using such procedure, the decrease in ethanol concentration in the culture media was completely avoided. Cultivating cells in ethanol-free media inside sealed plastic boxes did not change their viability, growth rate, protein and phospholipid composition of the cells or the pH of the media, compared to cultures grown outside the boxes.
本研究报告了一种在细胞培养基中随时间维持恒定乙醇浓度的模型的开发情况。当神经母细胞瘤×胶质瘤细胞（NG 108-15）在含有乙醇的培养基中于37℃培养箱的标准培养条件下生长时，24小时后观察到90%的蒸发率。为了抵消蒸发，将细胞培养皿与一个装有适量乙醇的开口培养皿一起放置在聚苯乙烯盒内。通过使用这种方法，完全避免了培养基中乙醇浓度的降低。与在盒外培养的细胞相比，在密封塑料盒内无乙醇培养基中培养细胞不会改变其活力、生长速率、细胞的蛋白质和磷脂组成或培养基的pH值。
