用于冷冻保存的糖与糖醇共熔混合物的特性研究
Characterization of eutectic mixtures of sugars and sugar-alcohols for cryopreservation.
作者信息
Joules Adam, Burrows Tessa, Dosa Peter, Hubel Allison
机构信息
Department of Biomedical Engineering, University of Minnesota, Minneapolis, 55455, USA.
Department of Medicinal Chemistry, Institute for Therapeutics Discovery and Development, University of Minnesota, Minneapolis, 55455, USA.
出版信息
J Mol Liq. 2023 Feb 1;371. doi: 10.1016/j.molliq.2022.120937. Epub 2022 Nov 29.
Natural Deep Eutectic Systems (NADES) composed of sugar and sugar alcohols have been studied and applied in a variety of biological applications. Understanding their interaction with water across dilution and temperature is inherently important for maximizing the utility of NADES. Herein a wide range of sugar:sugar-alcohol molar ratios were synthesized and characterized by viscosity, molar excess volume, differential scanning calorimetry, water activity, and confocal Raman cryomicroscopy. NADES were found to have greater viscosity, reduced heat of fusion, greater absolute molar excess volume, lower water activity, and stronger hydrogen bonding of water than non-NADES mixtures. This is hypothesized to be due to cumulatively stronger hydrogen bonding interactions between components in pure and diluted NADES with the strongest interactions in the water-rich region. This work provides useful data and further understanding of hydrogen bonding interaction strength for a wide range of molar ratios in pure to well-diluted forms.
由糖和糖醇组成的天然低共熔体系（NADES）已得到研究，并应用于多种生物学领域。了解它们在稀释和温度变化时与水的相互作用对于最大化NADES的效用至关重要。在此，合成了一系列糖与糖醇的摩尔比，并通过粘度、摩尔过量体积、差示扫描量热法、水分活度和共聚焦拉曼低温显微镜进行了表征。结果发现，与非NADES混合物相比，NADES具有更高的粘度、更低的熔化热、更大的绝对摩尔过量体积、更低的水分活度以及更强的水氢键。据推测，这是由于纯NADES和稀释后的NADES中各成分之间的氢键相互作用逐渐增强，且在富水区域相互作用最强。这项工作为纯态到高度稀释态的多种摩尔比提供了有用的数据，并进一步加深了对氢键相互作用强度的理解。
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