Hui Jian, Bai Haibo, Lyu Xuelian, Ma Sishuang, Chen Xiaojun, Li Shuhua
Ningxia Key Laboratory of Agricultural Biotechnology, Agricultural Biotechnology Research Center, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia, China.
Front Plant Sci. 2023 Jan 4;13:1067590. doi: 10.3389/fpls.2022.1067590. eCollection 2022.
Wheat is one of the most important food crops in the world and drought can severely impact on wheat productivity. The identification and deployment of genes for improved water use efficiency (WUE) can help alleviate yield loss under water limitation. In this study, a high-density genetic linkage map of wheat recombinant inbred lines (Ningchun 4 x Ningchun 27) containing 8751 specific locus amplified fragment (SLAF) tags (including 14757 SNPs), with a total map distance of 1685 cM and an average inter-marker map distance of 0.19 cM was constructed by SLAF-seq technology. The economic yield WUE and nine related traits under three water treatments was monitored over four years. The results showed that loci conditioning WUE were also associated with grain carbon isotope discrimination (CID), flag leaf chlorophyll content, plant height, 1000-grain weight, grain weight per spike and grain number per spike. One locus on chromosome 2B explained 26.3% WUE variation in multiple environments. Under good soil moisture conditions before flowering, the high CID genotype , was associated with WUE, high grain weight per spike, and kilo-grain weight. Under rain-fed conditions, the low CID genotype tended to maintain more spike number and was associated with improved WUE and yield. The introduction of good chromosome fragments of into elite lines by molecular marker assisted selection will boost up the cultivation of high-yield and water-saving wheat varieties.
小麦是世界上最重要的粮食作物之一，干旱会严重影响小麦的生产力。鉴定和利用提高水分利用效率（WUE）的基因有助于减轻水分限制条件下的产量损失。本研究利用SLAF-seq技术构建了一张包含8751个特异性位点扩增片段（SLAF）标签（包括14757个单核苷酸多态性，SNPs）的小麦重组自交系（宁春4号×宁春27号）高密度遗传连锁图谱，总图距为1685厘摩（cM），标记间平均图距为0.19 cM。连续四年监测了三种水分处理下的经济产量水分利用效率及九个相关性状。结果表明，调控水分利用效率的位点还与籽粒碳同位素分辨率（CID）、旗叶叶绿素含量、株高、千粒重、穗粒重和穗粒数有关。2B染色体上的一个位点在多个环境中解释了26.3%的水分利用效率变异。在开花前土壤水分良好的条件下，高CID基因型与水分利用效率、高穗粒重和千粒重相关。在雨养条件下，低CID基因型倾向于保持更多的穗数，并与提高的水分利用效率和产量相关。通过分子标记辅助选择将优良染色体片段导入优良品系，将促进高产节水小麦品种的培育。
