Oriaku E T, Soliman K F
College of Pharmacy and Pharmaceutical Sciences, Florida A & M University, Tallahassee.
Pharmacology. 1987;35(4):234-40. doi: 10.1159/000138315.
Male Sprague-Dawley rats weighing between 180 and 220 g and maintained under controlled lighting and temperature conditions were used in this experiment. Animals were given ethanol (3 g/kg, p.o.) 24 h after fasting. One group was given ethanol at 10.00 h (light phase) and the other at 22.00 h (dark phase). One hour later, the treated animals with their proper controls were sacrificed and the mucosa of the stomach, duodenum, ileum and colon were separated and assayed for choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities. Data obtained indicate that the administration of ethanol resulted in significant decline (p less than 0.01) in ChAT activity in the stomach and the colon during the light phase. A significant increase (p less than 0.01) in ChAT activity was also noted in the ileum during the dark phase. There was a significant decrease (p less than 0.01) in AChE activity in the stomach during the dark phase. The administration of ethanol also resulted in a significant decline in AChE activity (p less than 0.05) in the duodenum and the colon (p less than 0.01). The results obtained indicate that the gastrointestinal changes caused by ethanol administration may be related to changes in the cholinergic enzymes of the mucosa of the gastrointestinal tract.
本实验使用体重在180至220克之间、饲养于光照和温度受控条件下的雄性Sprague-Dawley大鼠。动物禁食24小时后给予乙醇（3克/千克，口服）。一组在上午10:00（光照期）给予乙醇，另一组在晚上22:00（黑暗期）给予乙醇。一小时后，将处理过的动物及其相应对照处死，分离胃、十二指肠、回肠和结肠的黏膜，测定胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）活性。所获数据表明，在光照期给予乙醇导致胃和结肠中的ChAT活性显著下降（p小于0.01）。在黑暗期，回肠中的ChAT活性也显著增加（p小于0.01）。在黑暗期，胃中的AChE活性显著降低（p小于0.01）。给予乙醇还导致十二指肠和结肠中的AChE活性显著下降（十二指肠p小于0.05，结肠p小于0.01）。所获结果表明，给予乙醇引起的胃肠道变化可能与胃肠道黏膜胆碱能酶的变化有关。
