Makia Raghda, Al-Sammarrae Khulood, Al-Halbosiy Mohammad, Al-Mashhadani Mohammed
Biotechnology College, Al-Nahrain University Baghdad, Iraq.
Department of Forensic Techniques, Al-Farahidi University, Baghdad, Iraq.
Rep Biochem Mol Biol. 2022 Oct;11(3):487-492. doi: 10.52547/rbmb.11.3.487.
Normally happening substances like flavonoids are regarded as active candidates for the treatment and prevention of cancer The purpose of this study was to see how Iraqi total flavonoid affected cell lines biologically and human lung fibroblast normal cell line (WISH).
Plant powder was extracted by reflex apparatus, then thin-layer chromatography (TLC) was used to determine total flavonoids. Cytotoxicity assay (MTT) was used to determine the cytotoxic activity of the prepared plant against human breast cancer (MCF-7), cells human cervix cancer (HELA), human colon cancer (Caco-2) and human lung fibroblast normal cell line (WISH).
The flavonoids Rutin, Quercetin, Kaempferol, and luteolin were detected using the Thin Layer Chromatography (TLC) technique. In contrast to the negative control, the extract inhibited cell growth to a highest of 82.158% for MCF-7 and 61.360% for Caco-2 at the concentration (100 µg/ml), and (54.880%) for Hela cell line at the concentration (100 µg/ml). In addition, the concentration (6.25 µg/ml) of total flavonoid extract produced a decrease in the growth of the normal WISH cell line to reach (1.094%).
contain high amounts of flavonoids, the qualification of some flavonoids compounds was detected using TLC. The total flavonoids showed significant cytotoxic activity against various types of cancer cell lines and normal cell line , the antitumor activity was highly efficient in a dose and cell type dependent manner.
像黄酮类化合物这样的天然存在物质被视为癌症治疗和预防的活性候选物。本研究的目的是观察伊拉克总黄酮对细胞系以及人肺成纤维细胞正常细胞系（WISH）的生物学影响。
通过回流装置提取植物粉末，然后用薄层色谱法（TLC）测定总黄酮。采用细胞毒性试验（MTT）来测定所制备植物对人乳腺癌（MCF - 7）细胞、人宫颈癌（HELA）细胞、人结肠癌（Caco - 2）细胞和人肺成纤维细胞正常细胞系（WISH）的细胞毒性活性。
使用薄层色谱（TLC）技术检测到了黄酮类化合物芦丁、槲皮素、山奈酚和木犀草素。与阴性对照相比，提取物在浓度为100μg/ml时对MCF - 7细胞的生长抑制率最高可达82.158%，对Caco - 2细胞为61.360%，对Hela细胞系在浓度为100μg/ml时为54.880%。此外，总黄酮提取物浓度为6.25μg/ml时，正常WISH细胞系的生长下降至1.094%。
含有大量黄酮类化合物，通过TLC检测到了一些黄酮类化合物。总黄酮对各种类型的癌细胞系和正常细胞系均表现出显著的细胞毒性活性，其抗肿瘤活性具有高度的剂量和细胞类型依赖性。
