Department of Experimental Oncology, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia.
J Med Food. 2010 Apr;13(2):452-9. doi: 10.1089/jmf.2008.0159.
In this study we investigated antioxidative and antiproliferative activity of different horsetail (Equisetum arvense L.) extracts. The antioxidative activity was measured by the electron spin resonance (ESR) spectroscopy-spin trapping method. The influence of different horsetail extracts during lipid peroxidation of (1) sunflower oil induced by the lipophilic azo-initiator 4,4'-azobis(4-cyanovaleric acid) and (2) soybean phosphatidylcholine liposomes induced by the hydrophilic azo-initiator 2,2'-azobis(2-amidinopropane) dihydrochloride was studied. Antiproliferative activity was measured using the sulforhodamine B colorimetric assay on the human cancer cell lines HeLa, HT-29, and MCF7. The results of ESR analysis confirmed that the extracts investigated suppressed the formation of lipid peroxyl radicals in both systems investigated in a dose-dependent manner. The results indicate that n-butanol, methanol, ethyl acetate, and water extracts had significant peroxyl radical scavenging activity. Extracts inhibited cell growth that was dependent on cell line, type of extract, and extract concentration. Ethyl acetate extract exhibited the most prominent antiproliferative effect, without inducing any cell growth stimulation on human tumor cell lines. The results obtained suggest that the horsetail extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals.
在这项研究中，我们研究了不同马尾草（Equisetum arvense L.）提取物的抗氧化和抗增殖活性。抗氧化活性通过电子自旋共振（ESR）光谱自旋捕获法测量。研究了不同马尾草提取物对（1）脂溶性偶氮引发剂 4,4'-偶氮双（4-氰基戊酸）诱导的葵花籽油和（2）亲水性偶氮引发剂 2,2'-偶氮双（2-脒基丙烷）二盐酸盐诱导的大豆磷酯酰胆碱脂质体的脂质过氧化的影响。使用人癌细胞系 HeLa、HT-29 和 MCF7 的磺基罗丹明 B 比色法测量抗增殖活性。ESR 分析的结果证实，所研究的提取物以剂量依赖的方式抑制了两种体系中脂氧自由基的形成。结果表明，正丁醇、甲醇、乙酸乙酯和水提取物具有显著的过氧自由基清除活性。提取物抑制细胞生长，这取决于细胞系、提取物类型和提取物浓度。乙酸乙酯提取物表现出最显著的抗增殖作用，对人肿瘤细胞系没有诱导任何细胞生长刺激。研究结果表明，马尾草提取物可用作天然抗氧化剂的易得来源和潜在的植物化学物质。
