Uttlová Petra, Urban Jan
National Reference Laboratory for Disinfection and Sterilization, National Institute of Public Health, Prague, Czech Republic.
Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Cent Eur J Public Health. 2022 Dec;30(4):230-234. doi: 10.21101/cejph.a7141.
The aim of the study was to investigate possible emergence of resistance to disinfectants in Bordetella pertussis strains isolated from patients with whooping cough in the Czech Republic in 2014 and 2015.
In an EN1500-based study, clean and dry fingertips of volunteers were always contaminated with one of the two clinical isolates of B. pertussis. Clinical isolates of B. pertussis were obtained from the National Reference Laboratory for Pertussis and Diphtheria, National Institute of Public Health (NIPH), Prague, Czech Republic. Dry and contaminated fingertips were immersed in 10 ml medium and then rubbed with the fingers for 1 minute. After that, the hands were treated with isopropanol 60% v/v or tested products, and then the fingertips were rubbed again into 10 ml of pure medium for 1 minute. The suspensions obtained were immediately diluted and plated on charcoal medium.
Ethanol-based product A and propanol-based product B showed bactericidal activity after 30 s of contact. The confidence interval limit for product A and B was 0.12 and 0.19, respectively. Quaternary ammonium compound-based product C was found to be ineffective after 30 s of contact. The confidence interval limit for product C was 0.62.
Products A and B were assessed as effective against clinical isolates of B. pertussis in accordance with EN 1500. Quaternary ammonium compound-based product C did not comply with the requirements of EN 1500.
本研究旨在调查2014年和2015年从捷克共和国百日咳患者中分离出的百日咳博德特氏菌菌株对消毒剂产生耐药性的可能性。
在一项基于EN1500的研究中，志愿者干净干燥的指尖总是被两种百日咳博德特氏菌临床分离株之一污染。百日咳博德特氏菌的临床分离株取自捷克共和国布拉格国家公共卫生研究所（NIPH）的百日咳和白喉国家参考实验室。将干燥且被污染的指尖浸入10毫升培养基中，然后用手指揉搓1分钟。之后，用60%（v/v）异丙醇或受试产品处理双手，然后将指尖再次在10毫升纯培养基中揉搓1分钟。立即将得到的悬液稀释并接种到活性炭培养基上。
乙醇基产品A和丙醇基产品B在接触30秒后显示出杀菌活性。产品A和B的置信区间限值分别为0.12和0.19。基于季铵化合物的产品C在接触30秒后被发现无效。产品C的置信区间限值为0.62。
根据EN 1500，产品A和B被评估为对百日咳博德特氏菌临床分离株有效。基于季铵化合物的产品C不符合EN 1500的要求。
