Shang Jinhua, Yu Shanshan, Li Ruomeng, He Yuqiu, Wang Yushi, Wang Fuan
Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430072, P. R. China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
Nano Lett. 2023 Feb 22;23(4):1386-1394. doi: 10.1021/acs.nanolett.2c04658. Epub 2023 Jan 31.
Rolling circle amplification (RCA) enables the facile construction of compact and versatile DNA nanoassemblies which are yet rarely explored for intracellular analysis. This is might be ascribed to the uncontrollable and inefficient probe integration/activation. Herein, by encoding with tandem allosteric deoxyribozyme (DNA-cleaving DNAzyme), a multifunctional RCA nanogel was established for realizing the efficient intracellular microRNA imaging via the successive activation of the RCA-disassembly module and signal amplification module. The endogenous microRNA stimulates the precise degradation of DNA nanocarriers, thus leading to the efficient exposure of RCA-entrapped DNAzyme biocatalyst for an amplified readout signal. Our bioorthogonal DNAzyme disassembly strategy achieved the robust analysis of intracellular biomolecules, thus showing more prospects in clinical diagnosis.
滚环扩增（RCA）能够轻松构建紧凑且通用的DNA纳米组装体，然而其在细胞内分析方面的应用却鲜有探索。这可能归因于探针整合/激活的不可控性和低效性。在此，通过串联变构脱氧核酶（切割DNA的脱氧核酶）进行编码，构建了一种多功能RCA纳米凝胶，以通过依次激活RCA拆解模块和信号放大模块来实现细胞内微小RNA的高效成像。内源性微小RNA刺激DNA纳米载体的精确降解，从而有效地暴露包裹在RCA中的脱氧核酶生物催化剂，以获得放大的读出信号。我们的生物正交脱氧核酶拆解策略实现了对细胞内生物分子的稳健分析，因此在临床诊断中显示出更大的前景。
