利用衣藻的实验进化研究全基因组复制。

Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas.

机构信息

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.

Department of Biology, Ghent University, Ghent, Belgium.

出版信息

Methods Mol Biol. 2023;2545:351-372. doi: 10.1007/978-1-0716-2561-3_18.

DOI:10.1007/978-1-0716-2561-3_18

PMID:36720822

Abstract

In this chapter, we present the use of Chlamydomonas reinhardtii in experiments designed to study the evolutionary impacts of whole genome duplication. We shortly introduce the algal species and depict why it is an excellent model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related Chlamydomonas experiment. These steps include strain selection, ploidy determination, different methods of making diplo- and polyploid Chlamydomonas cells, replication, culturing conditions, preservation, and the ways to quantify phenotypic and genotypic change.

摘要

在本章中，我们介绍了莱茵衣藻在旨在研究全基因组加倍对进化影响的实验中的应用。我们简要介绍了藻类物种，并描述了为什么它是实验进化的绝佳模型。随后，我们讨论了与设计与倍性相关的衣藻实验相关的最相关步骤和方法。这些步骤包括菌株选择、倍性确定、制作二倍体和多倍体衣藻细胞的不同方法、复制、培养条件、保存以及量化表型和基因型变化的方法。