Keshavarz Mohsen, Janati-Namin Nahid, Arjeini Yaser, Mokhtari-Azad Talat, Rezaei Farhad
The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2022 Aug;14(4):568-573. doi: 10.18502/ijm.v14i4.10244.
Parvovirus B19 (B19V) is usually transmitted through respiratory tract, but can also be received through blood transfusion. This study evaluated the seroprevalence, DNA existence, and circulating genotypes of B19V in hemophilia patients.
Serum samples of cases and controls were analyzed for B19V using ELISA and real-time PCR. Finally, obtained sequences were used for genotyping.
Among cases, 3% were anti-B19V IgM positive and 47% were anti-B19V IgG positive and B19V DNA was detected in 16% of them. However, among controls, 38% were anti-B19V IgG positive (P>0.05) and 5% were B19V DNA positive (P= 0.019). Also ∼13% of cases were positive and all of controls were negative for IgG avidity test (P= 0.029). Viral load in case group was higher than control group (P = 0.037).
Since hemophilia patients receive large amounts of blood factors, prevalence of B19V in these patients might be higher than normal subjects.
细小病毒B19（B19V）通常通过呼吸道传播，但也可通过输血感染。本研究评估了血友病患者中B19V的血清流行率、DNA存在情况及循环基因型。
采用酶联免疫吸附测定（ELISA）和实时荧光定量聚合酶链反应（real-time PCR）对病例组和对照组的血清样本进行B19V检测。最后，对获得的序列进行基因分型。
病例组中，3%抗B19V IgM阳性，47%抗B19V IgG阳性，16%检测到B19V DNA。然而，对照组中，38%抗B19V IgG阳性（P>0.05），5% B19V DNA阳性（P = 0.019）。此外，病例组约13% IgG亲和力试验阳性，对照组均为阴性（P = 0.029）。病例组病毒载量高于对照组（P = 0.037）。
由于血友病患者接受大量血液制品，这些患者中B19V的流行率可能高于正常人群。
