Javanmard Davod, Ziaee Masood, Ghaffari Hadi, Namaei Mohammad Hasan, Tavakoli Ahmad, Mollaei Hamidreza, Moghoofei Mohsen, Mortazavi Helya Sadat, Monavari Seyed Hamidreza
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
Blood Res. 2017 Dec;52(4):311-315. doi: 10.5045/br.2017.52.4.311. Epub 2017 Dec 26.
Human parvovirus B19 (B19V) is one of the smallest DNA viruses and shows great resistance to most disinfectants. Therefore, it is one of the common contaminant pathogens present in blood and plasma products. Parvovirus 4 (PARV4) is a newly identified parvovirus, which is also prevalent in parenteral transmission. In this study, we aimed to evaluate the prevalence of B19V and PARV4 DNA among patients with hemophilia in Birjand County in eastern Iran.
This was a cross-sectional epidemiological study comprising nearly all people with hemophilia in this region. Whole blood samples were taken after patient registration and sent for plasma isolation. After nucleic acid extraction, B19V was detected with real-time polymerase chain reaction, PARV4 DNA was then detected using sensitive semi-nested PCR.
In total, there were 86 patients with hemophilia, with mean age 28.5±1.5 years. Of these, 90.7% were men and 9.3% women; 84.9% had hemophilia A and 7.0% had hemophilia B. We found 11 patients (12.8%) were positive for B19V DNA and 8 were positive (9.3%) for PARV4 DNA. The prevalence of B19V was higher in middle-aged groups rather than younger people, whereas PARV4 infection was more common in younger patients ( <0.05).
There was a high prevalence of B19V and PARV4 infection in this high-risk group of patients with hemophilia. Due to the clinical significance of the B19 virus, imposing more precautionary measures for serum and blood products is recommended.
人细小病毒B19（B19V）是最小的DNA病毒之一，对大多数消毒剂具有很强的抵抗力。因此，它是血液和血浆制品中常见的污染病原体之一。细小病毒4（PARV4）是一种新发现的细小病毒，在肠道外传播中也很普遍。在本研究中，我们旨在评估伊朗东部比尔詹德县血友病患者中B19V和PARV4 DNA的流行情况。
这是一项横断面流行病学研究，纳入了该地区几乎所有的血友病患者。患者登记后采集全血样本并送去分离血浆。核酸提取后，用实时聚合酶链反应检测B19V，然后用灵敏的半巢式PCR检测PARV4 DNA。
共有86例血友病患者，平均年龄28.5±1.5岁。其中，男性占90.7%，女性占9.3%；84.9%为A型血友病，7.0%为B型血友病。我们发现11例患者（12.8%）B19V DNA呈阳性，8例患者（9.3%）PARV4 DNA呈阳性。B19V在中年组中的流行率高于年轻人，而PARV4感染在年轻患者中更常见（<0.05）。
在这个血友病高危患者群体中，B19V和PARV4感染的流行率很高。鉴于B19病毒的临床意义，建议对血清和血液制品采取更多预防措施。
