Levato Riccardo, Lim Khoon S
Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht 3584CT, The Netherlands.
Department of Orthopaedics, University Medical Center Utrecht, Utrecht University, Utrecht 3584CX, The Netherlands.
Biofabrication. 2023 Feb 1;15(2). doi: 10.1088/1758-5090/acb50f.
The integration of light-driven technologies into biofabrication has revolutionized the field of tissue engineering and regenerative medicine, with numerous breakthroughs in the last few years. Light-based bioprinting approaches (lithography, multiphoton and volumetric bioprinting) have shown the potential to fabricate large scale tissue engineering constructs of high resolution, with great flexibility and control over the cellular organization. Given the unprecedented degree of freedom in fabricating convoluted structures, key challenges in regenerative medicine, such as introducing complex channels and pre-vascular networks in 3D constructs have also been addressed. Light has also been proven as a powerful tool, leading to novel photo-chemistry in designing bioinks, but also able to impart spatial-temporal control over cellular functions through photo-responsive chemistry. For instance, smart constructs able to undergo remotely controlled shape changes, stiffening, softening and degradation can be produced. The non-invasive nature of light stimulation also enables to trigger such responses post-fabrication, during the maturation phase of a construct. Such unique ability can be used to mimic the dynamic processes occurring in tissue regeneration, as well as in disease progression and degenerative processes in vivo. Bringing together these novel multidisciplinary expertise, the present Special Issue aims to discuss the most recent trends, strategies and novel light-based technologies in the field of biofabrication. These include: 1) using light-based bioprinting to develop in vitro models for drug screening, developmental biology models, disease models, and also functional tissues for implantation; 2) novel light-based biofabrication technologies; 3) development of new photo-responsive bioinks or biomaterial inks.
将光驱动技术整合到生物制造中，已经彻底改变了组织工程和再生医学领域，在过去几年中取得了众多突破。基于光的生物打印方法（光刻、多光子和体积生物打印）已显示出制造高分辨率大规模组织工程构建体的潜力，在细胞组织方面具有极大的灵活性和可控性。鉴于在制造复杂结构方面拥有前所未有的自由度，再生医学中的关键挑战，如在三维构建体中引入复杂通道和预血管网络，也已得到解决。光也已被证明是一种强大的工具，不仅在设计生物墨水时能引发新的光化学，还能通过光响应化学对细胞功能进行时空控制。例如，可以制造出能够进行远程控制的形状变化、变硬、变软和降解的智能构建体。光刺激的非侵入性还能够在构建体成熟阶段制造后触发此类反应。这种独特的能力可用于模拟组织再生以及体内疾病进展和退化过程中发生的动态过程。汇集这些新颖的多学科专业知识，本期特刊旨在讨论生物制造领域的最新趋势、策略和基于光的新技术。这些包括：1）使用基于光的生物打印来开发用于药物筛选的体外模型、发育生物学模型、疾病模型以及用于植入的功能性组织；2）新颖的基于光的生物制造技术；3）新型光响应生物墨水或生物材料墨水的开发。
